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  4. Mouse Plasminogen(PLG) ELISA kit

Mouse Plasminogen(PLG) ELISA kit

  • 中文名称：
    小鼠纤维蛋白溶解酶原(PLG)酶联免疫试剂盒
  • 货号：
    CSB-EL018188MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        纤溶酶原（PLG）是一种酶原性蛋白，它能够转化为纤溶酶，参与机体的纤溶系统。在某些疾病状态下，PLG的水平可能会显著升高或降低，成为评估这些疾病的生物标志物之一。
        j9九游会登录入口首页生物所提供的Mouse Plasminogen(PLG) ELISA kit属于ELISA检测试剂盒，采用竞争法定量检测鼠血清、血浆、组织匀浆样本中的PLG，其灵敏度为15.63 ng/mL，检测范围为62.5 ng/mL-4000 ng/mL。
     
  • 别名：
    Plg ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 ng/mL-4000 ng/mL
  • 灵敏度：
    15.63 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 98    
    Range % 92-103    
    1:200 Average % 97    
    Range % 93-102    
    1:400 Average % 104    
    Range % 98-108    
    1:800 Average % 86    
    Range % 81-92    
  • 回收率：
    The recovery of mouse PLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 87 83-93    
    EDTA plasma (n=4) 94 88-98    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    4000 0.098 0.099 0.099      
    2000 0.125 0.132 0.129      
    1000 0.179 0.184 0.182      
    500 0.290 0.308 0.299      
    250 0.462 0.475 0.469      
    125 0.758 0.788 0.773      
    62.5 1.015 1.103 1.059      
    0 2.002 2.110 2.056      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    PLG在心血管疾病的研究中也备受关注，PLG参与了血管生成、动脉粥样硬化的形成、心肌损伤后修复等多个生理和病理过程。
  • 功能：
    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.; Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • 基因功能参考文献：
    1. These studies suggest a crucial role for peripheral PL in mediating neuroimmune cell activation and Alzheimer's disease (AD) progression and could provide a link to systemic inflammatory risk factors that are known to be associated with AD development. PMID: 30254165
    2. Variation in blood plasminogen levels, within the range seen in normal individuals, had marked effects on experimental ischemic brain injury PMID: 27319402
    3. plasmin protease activity prevents dystrophic calcification within injured skeletal muscle independent of its canonical fibrinolytic function. PMID: 27530373
    4. Plasminogen-deficient mice have extensive fibrin and neutrophil depositions in the wounded area long after re-epithelialisation, indicating inefficient debridement and chronic inflammation. Delayed formation of granulation tissue suggests that fibroblast function is impaired in the absence of plasminogen. PMID: 26791370
    5. findings identify plasmin as a decisive checkpoint in the inflammatory response during Macrophage activation syndrome (MAS) and a potential novel therapeutic target for MAS. PMID: 28325863
    6. Deficiency of plasminogen delays onset of autoimmune neuroinflammatory disease and protects from the development of paralysis. PMID: 28275164
    7. Plasminogen may regulate DPP-4 activity and glucose metabolism. PMID: 27592166
    8. Plasminogen-deficient mice frequently develop colon lesions and rectal prolapses. PMID: 26473342
    9. manganese transport protein C (MntC) is an extracellular matrix- and plasminogen-binding protein PMID: 25409527
    10. Plg from mouse plasma contains oxPtdPC adducts that are not affected by the action of Lp-PLA(2), suggesting that linkage to Plg protects oxPtdPCs from metabolism during their transport in the plasma. PMID: 21614211
    11. plasminogen-dependent proteolysis has a beneficial effect during neurological recovery after stroke. PMID: 24732409
    12. Pla-induced mononuclear cell recruitment in vivo was dependent on protease-activated receptor-1 activation of the MEK/ERK/NF-kappaB pathway, which led to the release of CCL2 and activation of CCR2. PMID: 25165151
    13. Plg may play an important role in innate immunity by changing expression of genes that contribute to phagocytosis. PMID: 24876560
    14. Plasminogen is a key molecular determinant of inflammatory joint disease capable of simultaneously driving or ameliorating arthritis pathogenesis in distinct anatomic locations in the same subject. PMID: 24574269
    15. Plasmin suppresses brain metastasis in two ways: by converting membrane-bound astrocytic FasL into a paracrine death signal for cancer cells, and by inactivating the axon pathfinding molecule L1CAM, which metastatic cells express for spreading along brain capillaries and for metastatic outgrowth. PMID: 24581498
    16. Plasminogen stimulates autocrine cytokine production in human airway smooth muscle cells in a manner mediated by plasmin and annexin A2. PMID: 23721211
    17. plasminogen contributes to angiogenesis related to macrophage accumulation, TGF-beta, and VEGF, thereby leading to the enhancement of bone repair. PMID: 23456978
    18. These findings highlight a critical role for plasmin in the catabolism of aggregated LDL by macrophages and provide a new context for considering the atherogenic role of plasmin. PMID: 23702659
    19. Gender affects skin wound healing in plasminogen deficient mice. PMID: 23527289
    20. The increased plasmin generation during the early stages of sepsis could cleave/inactivate TFPI and thus lead to thrombotic complications. PMID: 23106863
    21. Macrophage gene expression and foam cell formation are regulated by plasminogen. PMID: 23401155
    22. These results demonstrate the novel requirement of a-enolase for restoring homeostasis of injured muscle tissue, by controlling the pericellular localization of plasmin activity. PMID: 23239981
    23. In summary, these data indicate that plasmin-driven fibrinolysis facilitates tumor growth by maintaining patency of the tumor vasculature. PMID: 22815383
    24. Plasmin is essential in preventing periodontitis in mice PMID: 21704601
    25. found that male Plg knockout mice, but not their female counterparts, exhibited normochromic normocytic anemia PMID: 22056679
    26. Dengue virus (DENV)-immunized mouse sera demonstrates antibody binding to both DENV and Plg when compared with nonimmune control mouse serum. PMID: 22079981
    27. Plasmin plays an essential role in amplification of psoriasiform skin inflammation. PMID: 21311769
    28. in endothelial cells, plasmin specifically induces activation of conventional PKC, which phosphorylates serine 11 and serine 25 of A2, triggering dissociation of the (A2.p11)(2) tetramer. PMID: 21115493
    29. Plasminogen is highly expressed in specific populations of hippocampal, cortical, and hypothalamic neurons, and plasminogen-containing vesicles are mainly observed at neuronal somata. PMID: 21190118
    30. Plasminogen/plasmin modulates bone metabolism by regulating the osteoblast and osteoclast function. PMID: 21239499
    31. analysis of the generation and characterization of mice expressing a plasmin-inactivating active site mutation PMID: 20653841
    32. Data show that upon binding of plasminogen to neurons, plasmin formed by constitutively expressed tissue plasminogen activator degrades extracellular matrix proteins, leading to retraction of the neuron monolayer that detaches from the matrix. PMID: 19683575
    33. The role of Plg and alpha2-AP in vascular thrombolysis was studied in mice lacking Plg or a2-AP. Lack of Plg reduces the antithrombotic activities in vivo, but alpha2-AP plays a more important role in the formation/removal of venous thrombi. PMID: 11848464
    34. RT-PCR and In Situ Hybridization study of tissue distribution of Plg mRNA in adrenal, kidney, brain, testis, heart, lung, uterus, spleen, thymus and gut PMID: 11916082
    35. Plasmin activity is required for myogenesis in vitro and skeletal muscle regeneration in vivo. PMID: 11929773
    36. important role in adipose tissue accumulation associated with growth and differentiation of stromal cells PMID: 12083480
    37. These results indicate the presence of regulatory elements in the 5'-flanking region of the murine plasminogen promoter that may regulate murine plasminogen gene expression and, hence, plasmin activity. PMID: 12149246
    38. Plg mRNA was undetectable in the normal brain, but after kainate injection it was induced in neuronal cells in multiple, but specific areas. This distribution pattern coincided with that of the plasminogen activator inhibitor-2 mRNA. PMID: 12225871
    39. role of pCPB as a modulator of the pivotal functions of plasminogen in fibrinolysis and cell migration in vivo. PMID: 12417566
    40. Significantly greater retention of PMNs within the thrombi of PG(-/-) mice provided potential for more PMN derived fibrinolytic enzymes and a relatively greater role for PMNs-initiated fibrinolysis and differently cleaved fibrinogen degradation products. PMID: 12428098
    41. plasminogen directs the hepatocyte injury induced by transgene-derived urokinase-type plasminogen activator and mediates the reparative properties of urokinase-type plasminogen activator in the liver PMID: 12431907
    42. plasminogen mRNA from hepatocyte is dependent on the cell density and the stimulation by cell-cell contact may be an important factor for the constitutive expression of plasminogen gene PMID: 12559391
    43. Results demonstrate that the combination of matrix metalloproteinases and plasminogen is essential for the proper development of the placenta. PMID: 12900459
    44. plasmin has a role as an intracellular protease PMID: 12928439
    45. When uPA and uPAR transgenic mice were backcrossed into a plasminogen-deficient (Plg-/-) background, the dental and skin phenotypes appeared completely normal. PMID: 15161662
    46. Corneal angiogenesis is dependent on Plg. PMID: 15166500
    47. Plasminogen is bound to the cell surface by histidine-rich glycoprotein PMID: 15220341
    48. Plasminogen exerts a marked influence on both neutrophil and monocyte/macrophage recruitment to implanted biomaterials. PMID: 15456492
    49. Plg/Pla stimulates c-fos and egr-1 expression via activation of the MEK/ERK pathway PMID: 15721299
    50. Our results suggest that plasmin-mediated cell detachment could contribute to the pathological effects of Abeta in diseased brain. PMID: 15737740

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasminogen subfamily
  • 数据库链接：

    KEGG: mmu:18815

    STRING: 10090.ENSMUSP00000014578

    UniGene: Mm.971