Mouse Platelet-Derived Growth Factor Soluble Receptor α,PDGFsR-α ELISA kit

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

1. PDGFRalpha enrichment in hepatic stellate cell-derived extracellular vesicles drives liver fibrosis by a SHP2-dependent mechanism PMID: 29360139
2. PDGFR activity is evenly distributed at different membrane microdomains, while integrin-mediated signaling events have inhibitory effects on the activation of PDGFR specifically located in lipid rafts but not outside rafts. PMID: 28487538
3. downregulate growth signals provided by PDGFRalpha to initiate oligodendrocyte differentiation and myelination PMID: 30057274
4. PDGFRA is not essential for the derivation and maintenance of extraembryonic endoderm stem (XEN) cell lines . PMID: 28919262
5. loss of Pdgfra in endothelial-derived mesenchyme in the outflow tract endocardial cushions leads to a secondary defect in neural crest migration during development. PMID: 28714851
6. Investigated the developmental process of mesenchymal stem cells (MSCs) in embryos using the gene Pdgfra as a marker. We traced cells expressing Pdgfra and other genes (brachyury, Sox1 and Pmx1) in various mutant embryos until the adult stage. Embryonic MSCs emerge in waves and almost all adult bone marrow MSCs and white adipose tissue MSCs originate from mesoderm and embryonic Pdgfralpha-positive cells. PMID: 29378823
7. PDGFRalpha/PDGFRbeta signaling balance determines progenitor commitment to beige (PDGFRalpha) or white (PDGFRbeta) adipogenesis. PMID: 29158445
8. our data establish that aberrant stromal PDGFRalpha signaling disrupts extracellular matrix homeostasis during mammary gland development, resulting in increased mammary stiffness and increased potential for tumor growth. PMID: 28501760
9. Spreading of PDGFR-alpha-deficient lung fibroblasts was insensitive to increased rigidity, and their migration was not reduced by Rac1-guanine exchange factor (GEF)-inhibition. PDGFR-alpha-expressing fibroblasts migrated toward stiffer regions within two-dimensional substrates by increasing migrational persistence (durotaxis). PMID: 28775097
10. Histone H3.3K27M and Trp53 loss and PDGFRA overexpression accelerates disease onset and increases tumor invasion. PMID: 29107533
11. PDGFRalpha(+) cells represent the in vitro counterpart of in vivo PrE precursors, and their selection from cultured mESCs yields pure PrE precursors. PMID: 28089671
12. The diabetes-induced increase in PDGFRalpha+ cells may be mediated by FOXO3 up-regulation via the inhibition of the PI3K/Akt signaling pathway in STZ-induced diabetic mice. PMID: 28848093
13. Constitutive activation of PDGFRalpha leads to expansion of cartilage underlying the coronal sutures, which contribute to suture closure through endochondral ossification, in a process regulated in part by PI3K/AKT signaling. PMID: 28947535
14. OLIG2 modulates growth factor signaling in two distinct populations of glioma stem cells, characterized by expression of either the epidermal growth factor receptor (EGFR) or platelet-derived growth factor receptor alpha. PMID: 27626655
15. Conditional knockout of Pdgfra in Pdgfra-expressing tissues in mouse embryos at different embryonic days (E9.5 and E10.5) resulted in multiple developmental anomalies of the frontonasal region, the cranium and the abdominal wall musculature. Furthermore, the day at which the Pdgfra is deleted influences the repertoire of the anomalies of the conditional knockout embryos. PMID: 28934221
16. By analyzing mice with juxtamembrane or kinase domain point mutations that increase PDGFRalpha activity (V561D or D842V), it was found that PDGFRalpha activation inhibits embryonic white adipose tissue organogenesis in a tissue-autonomous manner. PMID: 28049691
17. the spatial location, mRNA expression and Immunophenotyping of PDGFRalpha(+) fibroblasts during sacculation and alveolarization, is reported. PMID: 28408205
18. FOXA3 is a marker of the Sertoli cell lineage and of the adult Leydig cell population, and is a regulator of Pdgfra transcription in Leydig cells. PMID: 28379539
19. in addition to representing a white adipose tisseu (WAT) adipogenic niche, different PDGFRalpha(+) cell subsets modulate obesity-induced WAT fibrogenesis and are associated with loss of metabolic fitness. PMID: 28215843
20. PDGFRalpha and PDGFRbeta are coexpressed in the craniofacial mesenchyme of mid-gestation mouse embryos and that ablation of Pdgfrb in the neural crest lineage results in increased nasal septum width, delayed palatal shelf development, and subepidermal blebbing. PMID: 27856617
21. The results from this study indicate that PDGF signaling is required for fiber hypertrophy, extracellular matrix production, and angiogenesis that occur during muscle growth. PMID: 28129672
22. polyadenylation of a PDGFRalpha intron in resident stem cells attenuates muscle fibrosis PMID: 27894125
23. transient middle cerebral-arterial occlusion (MCAO) was introduced into the mice with conditional Pdgfrb-gene inactivation, including N-PRbeta-KO mice where the Pdgfrb-gene was mostly inactivated in the brain except that in vascular pericytes PMID: 26435273
24. PDGFRalpha may be a relevant target to regulate connective tissue remodeling. PMID: 26639755
25. demonstrated that PDGFRalpha signaling promotes alveolar septation by regulating fibroblast activation and matrix fibroblast differentiation, whereas myofibroblast differentiation was largely PDGFRalpha independent PMID: 26414960
26. PDGFR alpha has an important role in cranial neural crest cell mitosis within the mandibular processes PMID: 26250625
27. PDGF signalling in the dermis and in dermal condensates is dispensable for hair follicle induction and formation. PMID: 25708924
28. PDGFRalpha has a role in demarcating the cardiogenic clonogenic Sca1+ stem/progenitor cell in adult murine myocardium PMID: 25980517
29. The present study provides new insights into the time-dependent commitment of the PDGFRalpha(+) cell lineage to lipofibroblasts and myofibroblasts during late lung development PMID: 26320158
30. Ganglioside GD3 enhances invasiveness of gliomas by forming a complex with PDGFRalpha and Yes Kinase. PMID: 25940087
31. PDGFRalpha targets progenitor cell plasticity as a profibrotic mechanism. PMID: 26019175
32. PDGFR is a key contributor to pulmonary vascular remodeling in experimental pulmonary hypertension. PMID: 25745058
33. Multipotential Neural precursors deficient in arylsulfatase A show a higher ratio of long versus short fatty acid sulfatides, reduction in PDGFRa, decreased AKT phosphorylation, and increased exosomal shedding of PDGFRa. PMID: 25605750
34. Pdgfralpha expression is important in the fibrotic response to liver injury PMID: 24667490
35. Identification of a PEDF signaling mechanism involving PPARG induction, suppression of Wnt/beta-catenin signaling and down-regulation of the PDGFRA/B. PMID: 24763086
36. SHP2 is required for cell transformation and ERK activation by mutant PDGF receptors. PMID: 24618081
37. The responses of colonic muscle PDGFRalpha(+) cells and smooth muscle cells to purines suggest that SMCs are unlikely targets for purinergic neurotransmission in colonic muscles. PMID: 25055825
38. Canonical Wnt-, Hh-, and Fgfr1/Fgfr2-signalling are dispensable for epicardial development, but Pdgfra-signalling is crucial for the differentiation of cardiac fibroblasts from epicardium-derived cells. PMID: 24000064
39. PDGFRalpha and IGF-1R are dynamically regulated and distributed in nucleus and cytoplasm in alveolar Rhabdomyosarcoma. PMID: 23928059
40. The HDAC11 transcript is a PDGF target; the HDAC11 mRNA abundance correlates inversely with proliferative status. PMID: 24047695
41. Temporal activation of PDGFRalpha in liver development is important in hepatic morphogenesis. PMID: 23529017
42. We thus establish PDGFRalpha as a novel regulator of MNP development and elucidate the roles of its downstream signaling pathways at cellular and molecular levels. PMID: 24086166
43. PDGFRalpha signaling in the primary cilium regulates NHE1-dependent fibroblast migration via coordinated differential activity of MEK1/2-ERK1/2-p90RSK and AKT signaling pathways. PMID: 23264740
44. the expression of PDGFRalpha and CD51 among bone marrow stromal cells characterizes a large fraction of Nestin cells, containing most fibroblastic CFUs, mesenspheres, and self-renewal capacity after transplantation PMID: 23776077
45. PDGFRalpha+ mesoderm is functionally significant in vascular development and hematopoiesis from phenotype analysis of genetically modified embryos. PMID: 23335233
46. These results suggest that an adaptive Src-Pdgfra-Raf-Mapk axis is relevant to PDGFRA inhibition in rhabdomyosarcoma. PMID: 22960170
47. PDGFR-alpha expression changes dramatically following lung volume reduction and a subpopulation of PDGF-alpha expressing fibroblasts differentiate into myofibroblasts. PMID: 22652199
48. Phf14 acts as a negative regulator of PDGFRalpha expression in mesenchymal cells undergoing normal and abnormal proliferation, and is a potential target for new treatments of lung fibrosis. PMID: 22730381
49. Platelet-derived growth factor receptor-alpha(+) cells had a spindle shape or stellate morphology and often possessed multiple processes that contacted one another forming a loose network PMID: 22151424
50. These findings demonstrate that transcriptional repression of PDGF-R-alpha after fibroblast injury involves paracrine activity of endogenous TNF-alpha, the formation of a c-Fos-YY1 complex, and negative regulatory activity by HDAC. PMID: 22322974

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Cell membrane; Single-pass type I membrane protein. Cell projection, cilium. Golgi apparatus.

Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily

Focally expressed in cortical interstitial cells and highly expressed in the interstitium of the papillary region. Also expressed by adventitial cells in arterial vessels. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstructi

KEGG: mmu:18595

STRING: 10090.ENSMUSP00000000476

UniGene: Mm.221403