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  4. Mouse Polymeric immunoglobulin receptor(PIGR) ELISA kit

Mouse Polymeric immunoglobulin receptor(PIGR) ELISA kit

  • 中文名称：
    小鼠聚合免疫球蛋白受体(PIGR)酶联免疫试剂盒
  • 货号：
    CSB-EL017981MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PIGR ELISA Kit was designed for the quantitative measurement of Mouse PIGR protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    Pigr; Polymeric immunoglobulin receptor; PIgR; Poly-Ig receptor
  • 缩写：
    PIGR
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PIGR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 90
    Range % 85-94
    1:200 Average % 89
    Range % 85-93
    1:400 Average % 84
    Range % 80-88
    1:800 Average % 102
    Range % 98-109
  • 回收率：
    The recovery of mouse PIGR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-100
    EDTA plasma (n=4) 91 85-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 1.925 1.867 1.896 1.757
    1000 1.366 1.282 1.324 1.185
    500 0.872 0.849 0.861 0.722
    250 0.551 0.546 0.549 0.410
    125 0.415 0.428 0.422 0.283
    62.5 0.284 0.291 0.288 0.149
    31.25 0.231 0.224 0.228 0.089
    0 0.142 0.135 0.139 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.; Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens. On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells.
  • 基因功能参考文献：
    1. pIgR/SIgA deficiency in the airways leads to persistent activation of innate immune responses to resident lung microbiota, driving progressive small airway remodelling and emphysema. PMID: 27046438
    2. Increased autoimmune diabetes in Pigr-deficient NOD mice is due to a "Hitchhiking" interval that refines the genetic effect of Idd5.4. PMID: 25835383
    3. the polymeric immunoglobulin receptor (pIgR) is highly expressed by renal cyst-lining cells. pIgR expression is, in part, driven by aberrant STAT6 pathway activation. PMID: 25922073
    4. pIgR on the blood-brain barrier endothelium may represent a novel pneumococcal adhesion receptor. PMID: 24841255
    5. Results reveal a novel Yes-EGFR-ERK-FIP5 signalling network for regulation of pIgA-pIgR transcytosis. PMID: 21037565
    6. Blockade of transepithelial transport of dimeric IgA in pIgR knockout mice reduces the degree of protection or cross-protection against viral infection, in parallel with a marked increase in serum IgA concentration and decrease in nasal IgA concentration. PMID: 11884464
    7. Mice deficient in SIgA have been generated by knocking out the pIgR and assessing their ability to maintain normal mucosal homeostasis and to induce mucosal and systemic immune responses against pathogenic and nonpathogenic stimuli. PMID: 12594279
    8. Mice that lack expression of the polymeric immunoglobulin receptor have approximately 5-fold more systemic IgA and 6-fold less nasal IgA antibodies than wild-type mice due to defective IgA transport into mucosal tissues. PMID: 15383591
    9. Data suggest that the pIgR and/or the secretory component are important for the maintenance of epithelial integrity and mucosal homeostasis in the colonic epithelium. PMID: 16911679
    10. pIgR is essential in intestinal defense against pathogenic microbes with high-level and persistent luminal presence. PMID: 17056558
    11. Novel localization of pIgR to the regulated secretory pathway of lacrimal gland acinar cells and its secretion therefrom may be affected by its novel interaction with Rab3D. PMID: 18171724
    12. The poly immunoglobulin receptor is the main pathway for IgA transport into the male reproductive tract. PMID: 18803626

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  • 亚细胞定位：
    [Polymeric immunoglobulin receptor]: Cell membrane; Single-pass type I membrane protein.; [Secretory component]: Secreted.
  • 数据库链接：