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Mouse Protein Wnt-2(WNT2) ELISA kit

  • 中文名称：
    小鼠Wnt-2蛋白(WNT2)酶联免疫试剂盒
  • 货号：
    CSB-EL026133MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse WNT2 ELISA Kit was designed for the quantitative measurement of Mouse WNT2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Wnt2 ELISA Kit; Irp ELISA Kit; Wnt-2 ELISA Kit; Protein Wnt-2 ELISA Kit; INT-1-related protein ELISA Kit; IRP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %83-87
    1:2Average %94
    Range %89-98
    1:4Average %103
    Range %97-108
    1:8Average %99
    Range %95-104
  • 回收率：
    The recovery of mouse WNT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-101
    EDTA plasma (n=4)8983-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.315 2.501 2.408 2.247
    5001.478 1.589 1.534 1.373
    2500.928 0.984 0.956 0.795
    1250.603 0.587 0.595 0.434
    62.50.325 0.340 0.333 0.172
    31.250.257 0.277 0.267 0.106
    15.60.201 0.215 0.208 0.047
    00.157 0.164 0.161
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

a. The active ingredient of the stop solution and its concentration ?
b. The type, concentration, and state (liquid/lyophilized) of the preservatives used. Please name the components with preservatives ?
c. If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
d. Expression host of the standards ?
e. Which components have H202 ( including other forms of peroxide such as urea hydrogen peroxide) ?Please list with the concentration of H202/peroxide in each, if any?

A:
Thanks for your inquiry!
CSB-EL026133MO
Stop solution is 4N sulfuric acid.
Preservatives is liquid proclin 300(1:3000)；
Standard is NS0-derived recombinant protein. It is lyophilized powder.
The concentration after reconstitution is 1000pg/mL（pls refer to Page 9 in the manual）；
The kit doesn't contain H2O2.
Pls let me know if you have any further questions. Thank you.
Q:

Just to clarify, Preservatives (proclin 300) is included only in the lyophilized standard ?
Also, please confirm that the only preservative is proclin 300? If not, please let me know the names and concentrations

A:
All components contain preservatives (proclin 300) except wash buffer、TMB Substrate and Stop Solution.

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons.
  • 基因功能参考文献：
    1. Transient expression of WNT2 promotes somatic cell reprogramming by inducing beta-catenin nuclear accumulation. PMID: 27211212
    2. Study indicates essential roles for Wnt2 and Wnt3 in chronic restraint stress-induced depression-like behaviors and antidepressant. PMID: 27622936
    3. Wnt 2 levels were markedly down-regulated by retinoic acid in pancreatic stellar cells and chronic pancreatitis. PMID: 26556479
    4. We propose that the WNT2/CTNNB1 pathway regulates CX43 expression and gap junction intercellular communication in granulosa cells by modulating CTNNB1 stability and localization in adherens junctions PMID: 23843235
    5. Pdgf signaling potentiates Wnt2-Wnt7b signaling to promote high levels of Wnt activity in mesenchymal progenitors that is required for proper development of endoderm-derived organs, such as the lung PMID: 22949635
    6. Expression of WNT2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. PMID: 22763454
    7. Wnt2 plays strong positive stage-specific role in cardiogenesis through non-canonical Wnt pathway in murine ES cells. PMID: 22146296
    8. show that Wnt2 signaling is necessary and sufficient for activation of a transcriptional and signaling network critical for smooth muscle specification and differentiation including myocardin/Mrtf-B and the signaling factor Fgf10 PMID: 21704027
    9. Wnt2 expression is downregulated at lung bud stage in the nitrofen model. Optical projection tomography is potentially a useful approach to visualize both gene expression and morphology during very early stages of lung development. PMID: 21034933
    10. The canonical WNT signaling pathway is intact in mouse granulosa cells and that WNT2 can act through beta-catenin, likely downstream of FZD9, to regulate DNA synthesis. PMID: 20107203
    11. Results identify Wnt2 as a novel regulator of dopaminergic progenitors and dopaminergic neuron development. PMID: 20018874
    12. Wnt2 works in a feedforward transcriptional loop with Gata6 to regulate posterior cardiac development. PMID: 20159597
    13. Upregulation by GATA6 in differentiating embryonal carcinoma cells is mediated by the direct binding of GATA-factor(s) to the Wnt2 promoter, suggesting Wnt2 is an immediate downstream target of GATA-factor regulation during early cardiogenesis. PMID: 16621466
    14. Wnt2 coordinates the commitment of mesoderm to hematopoietic, endothelial, and cardiac lineages in embryoid bodies PMID: 17098737
    15. FMR1, MECP2, and WNT2 expression were drastically down regulated in the Fragile X mouse brain. PMID: 19138667
    16. Study revealed new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. PMID: 19185060
    17. Data reveal that canonical Wnt2/2b signaling is required for the specification of lung endoderm progenitors in the developing foregut. PMID: 19686689

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    In embryos in the developing allantois, pericardium heart, and ventral-lateral mesoderm; in adults in lung, brain, heart and placenta.
  • 数据库链接：