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  4. Mouse Protein disulfide-isomerase A4(PDIA4) ELISA kit

Mouse Protein disulfide-isomerase A4(PDIA4) ELISA kit

  • 中文名称：
    小鼠蛋白质二硫键异构酶A4(PDIA4)酶联免疫试剂盒
  • 货号：
    CSB-EL017722MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PDIA4 ELISA Kit was designed for the quantitative measurement of Mouse PDIA4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Pdia4 ELISA Kit; Cai ELISA Kit; Erp72Protein disulfide-isomerase A4 ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; ER protein 72 ELISA Kit; ERp-72 ELISA Kit; ERp72 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %88
    Range %81-92
    1:2Average %100
    Range %93-104
    1:4Average %90
    Range %85-95
    1:8Average %99
    Range %91-103
  • 回收率：
    The recovery of mouse PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-96
    EDTA plasma (n=4)9589-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.654 2.543 2.599 2.417
    102.135 2.077 2.106 1.924
    51.345 1.332 1.339 1.157
    2.50.824 0.843 0.834 0.652
    1.250.526 0.545 0.536 0.354
    0.6250.359 0.369 0.364 0.182
    0.3120.278 0.266 0.272 0.090
    00.184 0.180 0.182
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. activated by IL11 in placenta PMID: 28487027
    2. PDIA4 as a negative regulator of cancer cell apoptosis. PMID: 28534513
    3. ERp72 functions separately from protein disulfide isomerase and ERp57 in supporting platelet aggregation. Defective aggregation of ERp72-null platelets was recovered by ERp72, but not other thiol isomerases. PMID: 28576878
    4. A network of endoplasmic reticulum ERp57, ERp72, and ERp29 facilitate polyomavirus infection. PMID: 21159867
    5. There were increased numbers of ERp72-immunoreactive cells in the dorsal and lateral cortex during sleep deprivation. PMID: 12535952
    6. These results suggest that domains 1 and 2 of ERp72 contribute to catalytic efficacy, and domain 3 to binding affinity. PMID: 16333982

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  • 亚细胞定位：
    Endoplasmic reticulum lumen. Melanosome.
  • 蛋白家族：
    Protein disulfide isomerase family
  • 数据库链接：

    KEGG: mmu:12304

    STRING: 10090.ENSMUSP00000076521

    UniGene: Mm.2442