Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Protein notum homolog(NOTUM) ELISA kit

Mouse Protein notum homolog(NOTUM) ELISA kit

  • 中文名称：
    小鼠notum蛋白同源物(NOTUM)酶联免疫试剂盒
  • 货号：
    CSB-EL015955MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse NOTUM ELISA Kit was designed for the quantitative measurement of Mouse NOTUM protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    NotumPalmitoleoyl-protein carboxylesterase NOTUM ELISA Kit; EC 3.1.1.98 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %109
    Range %104-113
    1:2Average %93
    Range %89-99
    1:4Average %100
    Range %96-104
    1:8Average %88
    Range %84-92
  • 回收率：
    The recovery of mouse NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9894-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.779 2.798 2.789 2.587
    52.201 2.156 2.179 1.977
    2.51.484 1.451 1.468 1.266
    1.250.845 0.899 0.872 0.670
    0.6250.555 0.561 0.558 0.356
    0.3120.432 0.445 0.439 0.237
    0.1560.312 0.326 0.319 0.117
    00.201 0.203 0.202
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
  • 基因功能参考文献：
    1. Notum influences mesenchymal cell differentiation by generating a barrier for Wnt ligands produced and secreted by airway epithelial cells to attenuate Wnt signaling. PMID: 29428562
    2. The findings support a significant role for NOTUM in modulating WNT signaling pathways that have pleiotropic effects on tooth and kidney development. PMID: 26926082
    3. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
    4. Male mice deficient in Notum specifically in the liver develop metabolic dysfunctions implicating Notum in the development of Type 2 diabetes. PMID: 26974334
    5. Describes the human ortholog, and analyzes both the human and mouse promoters. PMID: 18429952

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Pectinacetylesterase family, Notum subfamily
  • 组织特异性：
    Widely expressed. Expressed in lung, ovary, kidney, liver and brain. Not detected in thymus, heart, spleen, stomach, skeletal muscle and bone marrow.
  • 数据库链接：