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Mouse R-spondin-2(RSPO2) ELISA kit

  • 中文名称：
    小鼠R脊椎蛋白2(RSPO2)酶联免疫试剂盒
  • 货号：
    CSB-EL020551MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse RSPO2 ELISA Kit was designed for the quantitative measurement of Mouse RSPO2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    Rspo2R-spondin-2 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 2 ELISA kit; Cristin-2 ELISA kit; mCristin-2 ELISA kit; Roof plate-specific spondin-2 ELISA kit
  • 缩写：
    RSPO2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Developmental Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 97    
    Range % 92-102    
    1:2 Average % 91    
    Range % 85-98    
    1:4 Average % 90    
    Range % 88-93    
    1:8 Average % 100    
    Range % 96-104    
  • 回收率：
    The recovery of mouse RSPO2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 87-92    
    EDTA plasma (n=4) 106 103-110    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2000 2.522 2.633 2.578 2.472    
    1000 2.123 2.213 2.168 2.062    
    500 1.579 1.544 1.562 1.456    
    250 1.020 0.989 1.005 0.899    
    125 0.744 0.688 0.716 0.610    
    62.5 0.460 0.432 0.446 0.340    
    31.25 0.203 0.214 0.209 0.103    
    0 0.102 0.109 0.106 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form.
  • 基因功能参考文献：
    1. Data report that Rspo2, is highly expressed in the spinal motor neuron (SMN) and binds to its receptor Lgr5, at the neuromuscular junction (NMJ). Rspo2 enhances the LRP4/MuSK signaling via Lgr5 in an agrin-independent manner and promotes AChR clustering. In addition, the loss of Rspo2 in mice compromises AChR clustering, the ultrastructure of the NMJ, and neuromuscular signal transduction. PMID: 27328992
    2. Rspo2 activates Wnt/beta-catenin signaling to reduce Col2a1 and Sox9 and to facilitate differentiation of proliferating chondrocytes into hypertrophic chondrocytes in growth cartilage PMID: 27012200
    3. High resolution crystal structure of an ectodomain variant of human LGR5 (hLGR5ecto) complexed with a signalling competent fragment of mouse Rspo2. PMID: 26123262
    4. These findings suggest that R-Spondin2 may promote hepatic stellate cell activation by enhancing the canonical Wnt pathway. PMID: 24852883
    5. R-spondin2 is a key molecular link between infection and intestinal homoeostasis. PMID: 23695692
    6. R-spondin 2 homolog (Xenopus laevis) is identified as an essential regulator of Citrobacter rodentium infection susceptibility in mice PMID: 24005619
    7. Oocyte-derived R-spondin2 is a paracrine factor essential for primary ovarian follicle development PMID: 23407710
    8. Rspo2 is required to limit the number of outer hair cells to three rows and for optimal arrangement of peripheral nerve fibers in the developing cochlea. PMID: 23192966
    9. Rspo2 mutation in combination with Rspo3 mutation causes severe limb truncation. PMID: 22610508
    10. Recombinant R-spondin2 and Wnt3a up- and down-regulate novel target genes in C57MG mouse mammary epithelial cells. PMID: 22238613
    11. Results indicate that Rspo2 and Wnt1 have mutually distinct effects on mammary epithelial cell growth and these effects are context-dependent. PMID: 21732367
    12. Rspo2 is a mesenchyme-derived factor that plays critical roles in regulating branchial arch 1 patterning and morphogenesis through ectodermal-mesenchymal interaction and a novel genetic factor for cleft palate. PMID: 21237142
    13. The findings suggest that Rspo2 expression in the apical ectodermal ridge (AER) is required for AER maintenance likely by regulating Wnt/beta-catenin signaling. PMID: 17904116
    14. This study shows that Rspo2 is critical for maintenance of the AER and for growth and patterning in limb development. PMID: 18067586
    15. Lrp6-mediated R-spondin 2 signaling through the canonical Wnt pathway is required for normal morphogenesis of the respiratory tract and limbs. PMID: 18256198
    16. Wnt11 signals through beta-catenin, activating Rspo2 expression, which is then required for Wnt11-mediated osteoblast maturation. PMID: 19213727
    17. These findings indicate that Rspo2 regulates midfacial, limb, and lung morphogenesis during development through the Wnt/beta-catenin signaling. PMID: 19233133
    18. The Rspo2 mutants had markedly reduced potency relative to the wild-type protein,demonstrating for the first time specific amino acid residues in Rspos that are critical for beta-catenin signaling. PMID: 19385064

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    R-spondin family
  • 数据库链接：