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  4. Mouse R-spondin-4(RSPO4) ELISA kit

Mouse R-spondin-4(RSPO4) ELISA kit

  • 中文名称：
    小鼠R脊椎蛋白4(RSPO4)酶联免疫试剂盒
  • 货号：
    CSB-EL020553MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse RSPO4 ELISA Kit was designed for the quantitative measurement of Mouse RSPO4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 34.4 pg/mL-2200 pg/mL and the sensitivity is 8.6 pg/mL.

  • 别名：
    Rspo4R-spondin-4 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 4 ELISA kit; Cristin-4 ELISA kit; mCristin-4 ELISA kit; Roof plate-specific spondin-4 ELISA kit
  • 缩写：
    RSPO4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    34.4 pg/mL-2200 pg/mL
  • 灵敏度：
    8.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Developmental Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 87-95
    1:2 Average % 97
    Range % 93-102
    1:4 Average % 92
    Range % 85-96
    1:8 Average % 97
    Range % 95-101
  • 回收率：
    The recovery of mouse RSPO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 92-101
    EDTA plasma (n=4) 87 83-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2200 2.452 2.371 2.412 2.280
    1100 1.956 1.815 1.886 1.754
    550 1.208 1.182 1.195 1.063
    275 0.717 0.701 0.709 0.577
    137.5 0.425 0.413 0.419 0.287
    68.75 0.292 0.288 0.290 0.158
    34.4 0.196 0.192 0.194 0.062
    0 0.132 0.131 0.132 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    R-spondin family
  • 数据库链接：