Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Regenerating islet-derived protein 3-alpha(REG3A) ELISA kit

Mouse Regenerating islet-derived protein 3-alpha(REG3A) ELISA kit

  • 中文名称：
    小鼠再生胰岛衍生蛋白3α(REG3A）酶联免疫试剂盒
  • 货号：
    CSB-EL019548MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse REG3A ELISA Kit was designed for the quantitative measurement of Mouse REG3A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 别名：
    Reg3a ELISA Kit; Pap2 ELISA Kit; Regenerating islet-derived protein 3-alpha ELISA Kit; REG-3-alpha ELISA Kit; Islet of Langerhans regenerating protein 3 ELISA Kit; Lithostathine 3 ELISA Kit; Pancreatitis-associated protein 2 ELISA Kit; Regenerating islet-derived protein III-alpha ELISA Kit; Reg III-alpha) [Cleaved into: Regenerating islet-derived protein 3-alpha 16.5 kDa form; Regenerating islet-derived protein 3-alpha 15 kDa form] ELISA Kit
  • 缩写：
    REG3A
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 102
    Range % 98-106
    1:2 Average % 98
    Range % 96-104
    1:4 Average % 88
    Range % 86-95
    1:8 Average % 96
    Range % 91-102
  • 回收率：
    The recovery of mouse REG3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-97
    EDTA plasma (n=4) 88 84-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.360 2.512 2.436 2.311
    100 1.831 1.987 1.909 1.784
    50 1.252 1.254 1.253 1.128
    25 0.747 0.766 0.757 0.632
    12.5 0.473 0.512 0.493 0.368
    6.25 0.315 0.321 0.318 0.193
    3.12 0.219 0.217 0.218 0.093
    0 0.124 0.126 0.125 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
  • 基因功能参考文献：
    1. skin injury increases IL-36gamma via the activation of TLR3-SLUG-VDR axis and IL-36gamma induces REG3A to promote wound healing PMID: 28774595
    2. Reg3alpha is an extracellular matrix-targeted reactive oxygen species scavenger that binds the fibrin scaffold resulting from hepatocyte death during acute liver failure. PMID: 25938566
    3. Data show that regenerating islet-derived 3-alpha (REG3alpha) is a plasma biomarker of gastrointestinal tract graft-versus-host disease that can be combined with clinical stage and histologic grade to improve risk stratification of patients. PMID: 21979939
    4. Reg3alpha stimulates beta-cell replication, by activating Akt kinase and increasing the levels of cyclin D1/CDK4. PMID: 19343564
  • 相关疾病：
    Overexpressed during the acute phase of pancreatitis.
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Small intestine and pancreas.
  • 数据库链接：

    KEGG: mmu:19694

    STRING: 10090.ENSMUSP00000098829

    UniGene: Mm.2552