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  4. Mouse Translationally-controlled tumor protein(TPT1) ELISA kit

Mouse Translationally-controlled tumor protein(TPT1) ELISA kit

  • 中文名称：
    小鼠翻译调节肿瘤蛋白(TPT1)酶联免疫试剂盒
  • 货号：
    CSB-EL024134MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse TPT1 ELISA Kit was designed for the quantitative measurement of Mouse TPT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.37 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL.

  • 别名：
    Tpt1 ELISA Kit; Trt ELISA Kit; Translationally-controlled tumor protein ELISA Kit; TCTP ELISA Kit; 21 kDa polypeptide ELISA Kit; p21 ELISA Kit; p23 ELISA Kit
  • 缩写：
    TPT1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    9.37 pg/mL-600 pg/mL
  • 灵敏度：
    2.34 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-91
    1:2Average %93
    Range %86-98
    1:4Average %97
    Range %92-102
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of mouse TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-97
    EDTA plasma (n=4)9088-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    6002.836 2.672 2.754 2.633
    3002.099 2.184 2.142 2.021
    1501.494 1.483 1.489 1.368
    750.822 0.765 0.794 0.673
    37.50.498 0.461 0.480 0.359
    18.750.267 0.246 0.257 0.136
    9.370.186 0.178 0.182 0.061
    00.125 0.117 0.121 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please send the current manual.
e. Please send your reference data for mouse plasma samples.

A:
Thanks for your inquiry!
CSB-EL024134MO
This kit is designed agaisnt full length protein. Pls refer to: according to: https://www.uniprot.org/uniprot/P63028.
Capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is 293 cell-derived recombinant protein.
Pls find attached manual.
We tested undiluted serum and plasma samples before and the test value is 40pg/ml.

靶点详情

  • 功能：
    Involved in calcium binding and microtubule stabilization.
  • 基因功能参考文献：
    1. The biological effects of Na,K-ATPase and TCTP interaction are reviewed. PMID: 29867020
    2. that TPT1 knockdown altered the BECN1 interactome, a representative MTOR-independent pathway, to stimulate autophagosome formation PMID: 28409693
    3. The first crystal structure of murine HRF to 4.0A resolution, reveals a conserved fold. A model for the role of murine HRF in mast cell activation was created based on the human HRF structure. PMID: 29216544
    4. Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels. PMID: 28550308
    5. TCTP-mediated spindle dynamics play a key role in maintaining oocyte quality during postovulatory aging. Overexpression of TCTP is sufficient to prevent aging-associated abnormalities in mouse oocytes. PMID: 28476647
    6. Leads to the enhanced and previously un-described interaction of RACK1 and TCTP. PMID: 27816562
    7. These data suggest the presence of a novel oxidative-stress-handling pathway where the anti-p53 molecule fortilin augments the peroxidase PRX1 by protecting it against degradation and inactivation of the enzyme. PMID: 26726832
    8. results demonstrate that TCTP is a microtubule-associating protein required to regulate spindle microtubule dynamics during meiotic maturation in mouse oocytes. PMID: 26802898
    9. TCTP over-expression-induced RhoA activation indicates that silencing of cellular TCTP may offer therapeutic approaches for the regulation of hypertension by inhibiting RhoA/Rho kinase pathway and modulating vascular contractility. PMID: 24918292
    10. osteoclastogenic activity of translationally-controlled tumor protein (TCTP) with reciprocal repression of p21 PMID: 25263704
    11. Fortilin, induced in the proatherosclerotic microenvironment in macrophages, protects macrophages against Bax-induced apoptosis, allows them to propagate, and accelerates atherosclerosis. PMID: 24043250
    12. Overexpression of translationally controlled tumor protein increases the severity of atherosclerosis and hypertension in apolipoprotein E knock-out mice PMID: 22415346
    13. These findings suggest that TCTP might play an important role in the process of podocyte hypertrophy under diabetic conditions via the regulation of mTORC1 activity and the induction of cell-cycle arrest. PMID: 22311416
    14. The TCTP downregulates the Oct4 expression by binding the Sf1 site of Oct4 promoter in mouse pluripotent cells. PMID: 22281008
    15. TCTP may play a significant role in embryo implantation in mice PMID: 21865235
    16. knockdown of Tctp resulted in increased amounts of P53 accompanied by a 50% reduction in the efficiency of tumor mammosphere formation PMID: 22157679
    17. Identified a subset of IgE and IgG antibodies as TCTP(HRF)-interacting molecules in vitro. HRF was able to dimerize and bind to Igs via interactions of its N-terminal and internal regions with the Fab region of Igs. PMID: 22133880
    18. Data indicate that Hprt, Rpl13a and Tpt1 are a set of stably expressed reference genes for accurate gene expression normalization in myocardial infarction studies in mice. PMID: 21858224
    19. The Tpt1-Npm1 complex as a novel marker for highly proliferating cells, and offer further insight into the network that controls cellular fate. PMID: 20505363
    20. Results imply that TCTP levels influence the sensitivity of beta cells to apoptosis PMID: 21063673
    21. TCTP levels influence sensitivity to apoptosis PMID: 19901967
    22. Plk phosphorylates TCTP on 2 Ser residues in vitro & cofractionates with the majority of kinase activity toward TCTP in mitotic cell lysates. Phosphorylation decreases its microtubule-stabilizing activity & promotes increased MT dynamics after metaphase. PMID: 12167714
    23. seven putative Tpt1 genes with different chromosomal localizations were identified; analysis of sequence, genomic organization, and in vitro promoter activity. PMID: 12782126
    24. TCTP stabilizes Mcl-1 through interfering with Mcl-1's degradation by the ubiquitin-dependent proteasome degradation pathway and modulates its antiapoptotic activity. PMID: 15798198
    25. an N-terminal region of a cytosolic protein, TCTP, is required for its binding to Bcl-xL and for its antiapoptotic activity PMID: 15870695
    26. These results suggest that despite that TCTP is widely expressed in many tissues or cell types, it appears to regulate cell proliferation and survival in a tissue- or cell type-specific manner. PMID: 17475776
    27. Fortilin is an intracellular Ca2+ scavenger, protecting cells against Ca2+-dependent apoptosis by binding and sequestering divalent calcium ions (Ca2+) from the downstream Ca2+-dependent apoptotic pathways. PMID: 17705784
    28. TCTP antisense expression in CD25(high) regulatory T cells aggravates cuff-injured vascular inflammation PMID: 18789801
    29. In early phase of development, fortilin functions as an inhibitor of the bone morphogenetic protein pathway. PMID: 19364479
    30. Study demonstrated that TCTP acts as a cataractogenic factor through the repression of Na,K-ATPase activity and calcium mobilization in lens epithelial cells. PMID: 19479337
    31. Proton pump inhibitors exert anti-allergic effects by reducing TCTP secretion PMID: 19484128
    32. TCTP plays a very modest role in thymocyte development, but is critical for peripheral T cell maintenance and T cell receptor (TCR)-mediated cell proliferation. PMID: 19605695

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TCTP family
  • 数据库链接：