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  4. Mouse Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

Mouse Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

  • 中文名称：
    小鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)酶联免疫试剂盒
  • 货号：
    CSB-E04764m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        VEGFR-2/Flk-1（Vascular Endothelial Growth Factor Receptor-2 / Fetal Liver Kinase-1）是血管内皮生长因子（VEGF）的主要受体。VEGFR-2/Flk-1含量的变化与血管新生和疾病进程（如肿瘤）的发生密切相关。
        j9九游会登录入口首页生物所提供的Mouse Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆样本中的VEGFR-2/Flk-1，其灵敏度为1.95 pg/mL，检测范围为7.8 pg/mL-500 pg/mL。
     
  • 别名：
    Kdr ELISA Kit; Flk-1 ELISA Kit; Flk1 ELISA Kit; Vascular endothelial growth factor receptor 2 ELISA Kit; VEGFR-2 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fetal liver kinase 1 ELISA Kit; FLK-1 ELISA Kit; Kinase NYK ELISA Kit; Protein-tyrosine kinase receptor flk-1 ELISA Kit; CD antigen CD309 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 86    
    Range % 82-91    
    1:200 Average % 101    
    Range % 98-105    
    1:400 Average % 87    
    Range % 84-91    
    1:800 Average % 100    
    Range % 95-105    
  • 回收率：
    The recovery of mouse VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-94    
    EDTA plasma (n=4) 107 103-110    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    500 2.564 2.536 2.550 2.438    
    250 1.513 1.531 1.522 1.410    
    125 0.985 0.961 0.973 0.861    
    62.5 0.551 0.560 0.556 0.444    
    31.2 0.345 0.351 0.348 0.236    
    15.6 0.242 0.251 0.247 0.135    
    7.8 0.174 0.176 0.175 0.063    
    0 0.113 0.110 0.112 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    VEGFR-2/Flk-1是一种受体蛋白，全名为血管内皮生长因子受体-2/胚胎成纤维母细胞激素样激酶-1。它是血管内皮生长因子（VEGF）信号通路中的重要组成部分，参与血管生成、修复和再生等生理过程。VEGFR-2/Flk-1在肿瘤血管形成过程中也起到了重要作用。
  • 功能：
    Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
  • 亚细胞定位：
    Cell junction. Endoplasmic reticulum. Cell membrane.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome.; [Isoform 2]: Secreted.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
  • 组织特异性：
    Expressed in endothelial cells (at protein level). Detected in embryonic endothelial cells, as well as hematopoietic stem and progenitor cells. Detected in vascular endothelium. Expressed at high levels in adult heart, lung, kidney, brain and skeletal mus
  • 数据库链接：

    STRING: 10090.ENSMUSP00000109144

    UniGene: Mm.285