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  4. Mouse Vitamin K-dependent protein S(PROS1) ELISA kit

Mouse Vitamin K-dependent protein S(PROS1) ELISA kit

  • 中文名称：
    小鼠维生素K依赖性蛋白S(PROS1)酶联免疫试剂盒
  • 货号：
    CSB-EL018754MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PROS1 ELISA Kit was designed for the quantitative measurement of Mouse PROS1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

  • 别名：
    Pros1 ELISA Kit; ProsVitamin K-dependent protein S ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 ng/mL-400 ng/mL
  • 灵敏度：
    1.56 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 92  
    Range % 88-96  
    1:400 Average % 87  
    Range % 84-90  
    1:800 Average % 95  
    Range % 91-99  
    1:1600 Average % 97  
    Range % 92-102  
  • 回收率：
    The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 98 94-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    400 0.080 0.083 0.082    
    200 0.117 0.119 0.118    
    100 0.177 0.172 0.175    
    50 0.276 0.296 0.286    
    25 0.504 0.490 0.497    
    12.5 0.923 0.931 0.927    
    6.25 1.461 1.496 1.479    
    0 2.539 2.587 2.563    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
  • 基因功能参考文献：
    1. An inhibitory role for PROS1 on BM-derived LCs. Collectively. PMID: 29871951
    2. This study identifies a duple role for PROS1 in stem-cell quiescence and as a pro-neurogenic factor, and highlights a unique segregation of increased stem cell proliferation from enhanced neuronal differentiation. PMID: 27753164
    3. Mice overexpressing protein S showed significant improvements in blood glucose level, glucose tolerance, insulin sensitivity, and insulin secretion compared with wild-type counterparts. diabetic protein S transgenic mice developed significantly less severe diabetic glomerulosclerosis than controls. PMID: 27207541
    4. results support a causal relationship between the PS-K196E mutation and venous thrombosis PMID: 26251307
    5. By revealing that neural stem-like cells act within the SVZ neurogenic niche as phagocytes and that the ProS/MerTK path represents an endogenous regulatory mechanism for SVZ cell phagocytic activity PMID: 25308179
    6. Data indicate that activated T cells express Pros1. PMID: 23850380
    7. Results demonstrate that Protein S is a Mer ligand, and is active in Mer-driven phagocytosis in the retina. PMID: 23259948
    8. A self-regulatory mechanism of Toll-like receptor signalling through the suppression of Gas6 and ProS expression is described. PMID: 22043818
    9. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor1. PMID: 20348395
    10. Data show that the spectrum of bioactivities of recombinant murine protein S with mouse plasma and smooth muscle cells is similar to that of human protein S. PMID: 19815836
    11. results demonstrate that ProS is a pleiotropic anticoagulant with activated Protein C-independent activities and highlight new roles for ProS in vascular development and homeostasis PMID: 19729839
    12. Pregnancy causes a decrease in APC resistance in mice, which can be explained by the elevation of protein S levels and increased TFPI activity in plasma. PMID: 19036061

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Plasma.
  • 数据库链接：