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  4. Mouse WAP four-disulfide core domain protein 2(WFDC2) ELISA kit

Mouse WAP four-disulfide core domain protein 2(WFDC2) ELISA kit

  • 中文名称：
    小鼠WAP四-二硫核心结构域蛋白2(WFDC2)酶联免疫试剂盒
  • 货号：
    CSB-EL026092MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse WFDC2 ELISA Kit was designed for the quantitative measurement of Mouse WFDC2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    Wfdc2 ELISA Kit; He4 ELISA Kit; WAP four-disulfide core domain protein 2 ELISA Kit; WAP domain-containing protein HE4 ELISA Kit
  • 缩写：
    WFDC2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse WFDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 81-95  
    1:2 Average % 96  
    Range % 90-102  
    1:4 Average % 97  
    Range % 92-105  
    1:8 Average % 95  
    Range % 89-98  
  • 回收率：
    The recovery of mouse WFDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 92 86-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.045 2.144 2.095 1.943  
    2000 1.624 1.743 1.684 1.532  
    1000 1.053 1.072 1.063 0.911  
    500 0.673 0.663 0.668 0.516  
    250 0.342 0.351 0.347 0.195  
    125 0.274 0.283 0.279 0.127  
    62.5 0.229 0.235 0.232 0.080  
    0 0.152 0.151 0.152 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have a few questions on product CSB-EL026092MO ：
1. Which is this standard in this kit, natural material or recombinant?
If recombinant, please let us know expression method(E.coli etc.).
2. Is the standard or antibody, including any additive agent(antiseptic
agent or preserving agent)? If so, please let us know composition and
concentration.
3. Is this product including Stop solution? If so, please tell us
composition and concentration for stop solution.
4. Please let us know whether this kit include any hazardous
substances(azide, thimerosal, 2-ME, etc). If so, please let us know
composition and concentration.

A:
Thanks for your inquiry!
Pls check our answer:
1, Standard of CSB-EL026092MO is NS0-derived recombinant protein
2,Preservative of this kit is proclin 300(1:3000)
3, Stop solution: 4N sulfuric acid
4, There is no hazardous substances (azide, thimerosal, 2-ME, etc) included in the kit.

靶点详情

  • 功能：
    Broad range protease inhibitor.
  • 基因功能参考文献：
    1. Causal testing implicates Wfdc2 and Hdac3 as novel genes affecting high density lipoprotein levels PMID: 21807117
  • 亚细胞定位：
    Secreted.
  • 数据库链接：