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  4. Mouse Xanthine Oxidase(XO) ELISA Kit

Mouse Xanthine Oxidase(XO) ELISA Kit

  • 中文名称：
    小鼠黄嘌呤脱氢酶/氧化酶(XDH)酶联免疫试剂盒
  • 货号：
    CSB-EL026189MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse XDH ELISA Kit was designed for the quantitative measurement of Mouse XDH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

  • 别名：
    Xdh ELISA Kit; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase ELISA Kit; XD ELISA Kit; EC 1.17.1.4); Xanthine oxidase ELISA Kit; XO ELISA Kit; EC 1.17.3.2 ELISA Kit; Xanthine oxidoreductase ELISA Kit; XOR)] ELISA Kit
  • 缩写：
    XDH
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse XDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1000 Average % 91    
    Range % 87-95    
    1:2000 Average % 98    
    Range % 94-102    
    1:4000 Average % 92    
    Range % 87-98    
    1:8000 Average % 92    
    Range % 88-97    
  • 回收率：
    The recovery of mouse XDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 101 97-104    
    EDTA plasma (n=4) 104 96-108    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    500 2.654 2.516 2.585 2.492    
    250 1.824 1.714 1.769 1.676    
    125 1.165 1.126 1.146 1.053    
    62.5 0.643 0.653 0.648 0.555    
    31.2 0.387 0.365 0.376 0.283    
    15.6 0.224 0.239 0.232 0.139    
    7.8 0.159 0.155 0.157 0.064    
    0 0.094 0.092 0.093 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species.
  • 基因功能参考文献：
    1. Its anti-hyperuricemic mechanisms may be ascribed to its inhibition of XOD and its up-regulation of organic anion transporter 1 (OAT1) and down-regulation of glucose transporter 9 (GLUT9). PMID: 30336599
    2. Our results demonstrated that Ripk3 was strongly upregulated in murine hearts subjected to IR injury and cardiomyocytes treated with LPS and H2O2..the present study helps to elucidate how necroptosis is mediated by ER stress, via the calcium overload /XO/ROS/mPTP opening axis PMID: 29502045
    3. eNOS deficiency is associated with an upregulation of XOR facilitating the nitrate-nitrite-NO pathway and decreasing the generation of ROS. This interplay between XOR and eNOS is proposed to play a significant role in NO homeostasis and blood pressure regulation. PMID: 27609225
    4. Collectively, these results demonstrate that XO inhibition protects against WD-induced vascular oxidative stress, fibrosis, impaired vasorelaxation, and aortic stiffness in females. Furthermore, excessive oxidative stress resulting from XO activation appears to play a key role in mediating vascular dysfunction induced by chronic exposure to WD consumption in females. PMID: 28539355
    5. NLRP3 inflammasome is decreased and IL-1beta secretion is inhibited in macrophages by hydroxysafflor yellow A and xanthine oxidase PMID: 27433030
    6. Extracts from Corylopsis coreana Uyeki (Hamamelidaceae) flos inhibit xanthine oxidase activity and prevent hyperuricemia. PMID: 27696407
    7. Our results suggest that a possible relationship between xanthine oxidase-related reactive oxygen species and TRPV1 may exist during the events preceding eccentric exercise PMID: 26987748
    8. inflammasome activation accompanied by an increase in xanthine oxidoreductase activity contributed to fat pad inflammation followed by osteoarthritis progression PMID: 26903297
    9. XOR is abundant in wounds and participates in normal wound healing through effects on ROS production. PMID: 25879627
    10. XOR regulated macrophage IL-1b secretion upon NLRP3 inflammasome activation via xanthine oxidase derived reactive oxygen species. PMID: 25800347
    11. Xanthine oxidase inhibitors are potentially potent therapies for patients with NASH, particularly that associated with hyperuricemia. PMID: 25999428
    12. these data support a novel function of the XO-NFAT5 axis in macrophage activation and TLR-induced arthritis PMID: 25044064
    13. The expression and the activity of XO were increased dramatically within the central nervous system of experimental autoimmune encephalomyelitis mice as compared to naive mice. PMID: 23951137
    14. Reduction in XOR gene expression in mice augments lipid accumulation in adipocytes, accompanied by an increase in oxidative stress, and induces obesity with insulin resistance in older age. PMID: 24158515
    15. Rat Xdh is a moonlighting protein that has two distinct catalytic activities as a dehydrogenase and an oxidase. PMID: 15878860
    16. Mouse Xdh is a moonlighting protein that has two distinct catalytic activities and acts a structural protein in milk fat droplets. PMID: 12502743
    17. In conclusion, CS increases XOR expression, and the enzyme is both sufficient and necessary for p53 induction and CS-induced EC apoptosis. PMID: 23380026
    18. we have established a mouse model for autosomal recessive early-onset renal failure due to a nonsense mutation in Xdh that is a model for xanthinuria in man. PMID: 23024809
    19. These findings provide new insight into XOR functions and demonstrate the power of untargeted metabolite profiling for systemic discovery of direct and indirect consequences of gene mutations and drug treatments. PMID: 22723833
    20. Xanthine oxidoreductase is involved in macrophage foam cell formation and atherosclerosis development. PMID: 22095983
    21. Age-related changes in xanthine oxidase activity and lipid peroxidation, as well as in the correlation between both parameters, in plasma and several organs from female mice PMID: 21626205
    22. These results show that XOR is an important functional component of differentiation whose diminished expression contributes to breast cancer aggressiveness PMID: 21775420
    23. The xanthine oxidoreductase expression in tissues is enhanced in germ free mice and this may explain the apparently greater tissue nitrate reductase activity observed in these animals. PMID: 20142047
    24. Xanthine oxidase (XO) activity contributes to the increased superoxide anion detected within the extracellular space of skeletal muscles during nondamaging contractile activity. PMID: 19828843
    25. the expression and membrane association of XOR in the mammary gland are tightly regulated by secretory activity, and the apical membrane association of XOR regulates the coupling of lipid to the plasma membrane during milk lipid secretion PMID: 12456835
    26. Deficiency of NOS1 leads to profound increases in XOR-mediated O(2)(.-) production, which in turn depresses myocardial excitation-contraction coupling. PMID: 15486091
    27. XOR activity is an endogenous physiological regulator of COX-2 expression. PMID: 15528468
    28. Stress activation of mammary epithelial cell Xdh is mediated by p38 MAPK and CCAAT-enhancer-binding protein-beta. PMID: 16452486
    29. We conclude that XOR enzymatic activity is significantly increased by mechanical stress via activation of p38 MAP kinase and ERK and plays a critical role in the pathogenesis of pulmonary edema associated with VILI. PMID: 16632522
    30. XOR is a novel regulator of adipogenesis and of PPARgamma activity and essential for the regulation of fat accretion. PMID: 17276354
    31. Although, numerous free radical generating systems may contribute to the postanoxic microglial cell damage, the xanthine-oxidase-mediated oxidant production seems to be of major importance. PMID: 17364940
    32. Alveolar cell apoptosis is dependent on p38 MAP kinase-mediated activation of xanthine oxidoreductase in ventilator-induced lung injury. PMID: 18669934
    33. XOR derived ROS mediated the migratory effect and also modulated COX-2 and MMP levels and function. PMID: 18767115
    34. Nox2-containing NADPH oxidase and xanthine oxidase are sources of superoxide in mouse trachea. PMID: 19076165
    35. PRY/SPRY/B30.2 domain of butyrophilin 1A1 (BTN1A1) binds to xanthine oxidoreductase PMID: 19531472
    36. XOR gene disruption induced the depletion of uric acid and the accumulation of triglyceride-rich substances, xanthine, and hypoxanthine in the renal tubules. PMID: 19667249

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  • 亚细胞定位：
    Cytoplasm. Peroxisome. Secreted.
  • 蛋白家族：
    Xanthine dehydrogenase family
  • 数据库链接：