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  4. Mouse apolipoprotein H (Apo-H) ELISA Kit

Mouse apolipoprotein H (Apo-H) ELISA Kit

  • 中文名称：
    小鼠载脂蛋白H(Apo-H)酶联免疫试剂盒
  • 货号：
    CSB-E10074m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse APOH ELISA Kit was designed for the quantitative measurement of Mouse APOH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

  • 别名：
    Apoh ELISA kit; B2gp1Beta-2-glycoprotein 1 ELISA kit; APC inhibitor ELISA kit; Activated protein C-binding protein ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 88  
    Range % 83-92  
    1:2000 Average % 97  
    Range % 91-101  
    1:4000 Average % 89  
    Range % 84-92  
    1:8000 Average % 97  
    Range % 91-101  
  • 回收率：
    The recovery of mouse Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-95  
    EDTA plasma (n=4) 103 98-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.669 2.625 2.647 2.512  
    500 2.180 2.112 2.146 2.011  
    250 1.611 1.600 1.606 1.471  
    125 0.859 0.831 0.845 0.710  
    62.5 0.515 0.527 0.521 0.386  
    31.2 0.321 0.333 0.327 0.192  
    15.6 0.248 0.242 0.245 0.110  
    0 0.136 0.134 0.135    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
  • 基因功能参考文献：
    1. During the parasite's hepatic replication, the C-terminal region of EXP-1 binds ApoH. This molecular interaction plays a pivotal role for successful Plasmodium liver-stage development. Down-regulation of ApoH expression in either hepatic cells or mouse livers by RNA interference resulted in impaired intrahepatic development. PMID: 28137845
    2. A1-A1, which is a soluble analog of ApoER2 that binds pathological beta2GPI/anti-beta2GPI complexes, has a positive impact on hemodynamics in lupus-prone mice with spontaneous anti-beta2GPI antibodies and hypertension PMID: 26990752
    3. These results suggested that CRP/oxidized low density lipoprotein/beta 2-Glycoprotein I complexes may induce lipid accumulation and inflammation in macrophages via the p38/MAPK and NFkappaB signaling pathways. PMID: 27512978
    4. TLR2 increase the stimulating effect of beta2GP1/anti-beta2GP1 complex on the expression of TNF-alpha in mouse peritoneal macrophages PMID: 27053607
    5. The Fifth Domain of Beta 2 Glycoprotein I Protects from Natural IgM Mediated Cardiac Ischaemia Reperfusion Injury. PMID: 27031114
    6. These results indicate that both NF-kappaB and c-Jun/AP-1 are involved in regulating anti-beta2GPI-induced expression of prothrombotic and proinflammatory molecules in vivo. PMID: 26829121
    7. Phospholipid scramblase 1 regulates phosphatidylserine exposure in response to oxygen stress, leading to beta2-glycoprotein I and IgM binding and lipid-mediated, inflammatory responses. PMID: 26216936
    8. beta2-GPI-D-I-tDCs may offer a novel approach for developing therapy for APS patients PMID: 24972993
    9. Data suggest that factors enabling a beta2-glycoprotein I (beta2GPI)-reactive T cell response may predispose individuals to the development of systemic lupus erythematosus (SLE)-related autoantibodies PMID: 25555913
    10. Deletion of beta2 GPI accelerates and potentiates the autoimmune phenotype in male BXSB.Yaa mice PMID: 24692206
    11. beta2-glycoprotein I-derived peptides attenuate injury and inflammation when administered in a therapeutic manner in intestinal ischemia/reperfusion injury. PMID: 23034168
    12. Interaction of beta2-glycoprotein I with lipopolysaccharide leads to Toll-like receptor 4 (TLR4)-dependent activation of macrophages PMID: 21965665
    13. beta2GPI binding to EC requires priming with pro-inflammatory factors which is not needed for uterine and placental localization probably dependent on hormonal changes. PMID: 21791419
    14. gene knock-out mice display enhanced B16 melanoma development and tumor microvessel density PMID: 20655705
    15. Endothelial cell incubation with anti-lysobisphosphatidic-acid MoAb or with patients' IgG resulted in antibody binding to late endosomes and caused beta2GPI redistribution and accumulation in perinuclear vesicular structures reminiscent of late endosomes. PMID: 12008959
    16. Data are consistent with pathogenic mechanisms in antiphospholipid syndrome involving both neutralization of beta(2)-glycoprotein I (beta(2)GPI) function and beta(2)GPI-immunoglobulin complex formation. PMID: 15100383
    17. beta-2-glycoprotein 1 plays an important physiologic role in the recognition of dying cells PMID: 15843904
    18. certain subpopulations of antibodies raised against tetanus toxoid and cross-reactive with beta(2)GPI, because of the molecular mimicry mechanism, could have pathologic potential PMID: 19086991
    19. These results are compatible with a role for apolipoproteins in lipid metabolism and transport in the developing lung in association with the sex difference in surfactant lipid synthesis. PMID: 19106236

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    KEGG: mmu:11818

    STRING: 10090.ENSMUSP00000000049

    UniGene: Mm.2266