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  4. Mouse basic fibroblast growth factor,bFGF ELISA Kit

Mouse basic fibroblast growth factor,bFGF ELISA Kit

  • 中文名称：
    小鼠碱性成纤维细胞生长因子(bFGF)酶联免疫试剂盒
  • 货号：
    CSB-E08001m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    CUSABIO's bFGF mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse bFGF in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses an antibody specific for mouse bFGF coated on a 96-well plate. bFGF present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated bFGF antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

    bFGF, also called FGF2, is a powerful angiogenic molecule that stimulates smooth muscle cell growth, wound healing, and tissue repair. In addition, FGF2 may stimulate hematopoiesis and may play an important role in the differentiation and/or function of the nervous system, eyes, and skeleton. Together with heparin or heparin sulfate proteoglycan, FGF2 activates FGFRs, inducing its pleiotropic effects in different tissues and organs, including potent angiogenic effects and important roles in the differentiation and function of the central nervous system (CNS). FGF2 plays a critical role in the development of the CNS. FGF2/FGFR1 signaling has been a hotspot of therapeutic development for neurodegenerative diseases such as multiple sclerosis, Alzheimer’s disease, and Parkinson’s disease.

  • 别名：
    Fgf2 ELISA Kit; Fgf-2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %89-100
    1:2Average %94
    Range %90-99
    1:4Average %97
    Range %92-103
    1:8Average %94
    Range %87-98
  • 回收率：
    The recovery of mouse bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-95
    EDTA plasma (n=4)9692-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.895 2.863 2.879 2.711
    1002.455 2.364 2.410 2.242
    501.995 1.976 1.986 1.818
    251.556 1.532 1.544 1.376
    12.51.011 0.968 0.990 0.822
    6.250.452 0.427 0.440 0.272
    3.120.292 0.284 0.288 0.120
    00.169 0.167 0.168 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 基因功能参考文献：
    1. expressed in tissues of the female reproductive tract; affects sperm motility and acrosomal exocytosis PMID: 29866768
    2. present study highlights the central role for FGF-2 in the progression and maintenance of newly acquired blood and lymphatic vessels in the cornea of HSV-1-infected mice in the absence of infectious virions PMID: 28378806
    3. NF-kappaBmiR15abFGF/VEGFA axis contributes to the impaired angiogenic capacity of bone marrowmesenchymal stem cells in high fat dietfed mice. PMID: 28944834
    4. Knockout of the 18-kDa FGF-2 isoform significantly attenuated atherogenesis, reduced aortic plaques, reduced macrophage infiltration and suppressed oxidative stress in mice fed with a high fat diet at all-time points. PMID: 29466783
    5. Data show that exostosin-like 2 (EXTL2) controls fibroblast growth factor 2 (FGF2) signaling through regulation of heparan sulfate biosynthesis. PMID: 29305908
    6. FGF2 signaling can regulate osteoblastic niche cells to support HSC homeostasis in response to bone marrow damage PMID: 28662672
    7. Dynamic changes in heparan sulfate during muscle differentiation and ageing regulate myoblast cell fate and FGF2 signaling. PMID: 27496348
    8. altered glycosaminoglycans (GAG) distribution in mucopolysaccharidoses I (MPS I) chondrocytes, and altered GAG, FGF2 and Indian hedgehog distribution in growth plates from MPS I mice, is reported. PMID: 27105565
    9. Data show that LOX-PP enhances adipogenesis at least partially through inhibition of FGF-2 receptor signaling. PMID: 28452589
    10. tissue engineered periosteum can deliver FGF-2, TGF-beta1, and ASCs to a mouse critical-sized femur defect and further optimization may yield improved bone allograft healing. PMID: 27874253
    11. Thus, FGF-2 levels in hESCs culture systems can be manipulated to generate cells with longer telomere which would be advantageous in the applications of hESCs in regenerative medicine. PMID: 27757766
    12. Low FGF2 expression is associated with cardiac ischemia and systolic dysfunction. PMID: 28771625
    13. Mechanical strain stimulates vasculogenesis of embryonic stem cells by the intracellular messengers ROS, NO and calcium as well as by upregulation of angiogenesis guidance molecules and the angiogenic growth factors VEGF, FGF-2 and PDGF-BB. PMID: 27725190
    14. data suggest that FGF2 levels are critically related to anxiety behavior and hypothalamic pituitary- adrenal axis activity, likely through modulation of hippocampal glucocorticoid receptor expression, an effect that is likely receptor mediated, albeit not by FGFR1, FGFR2, and FGFR3. PMID: 27133954
    15. The differentiation of ERF-overexpressing trophoblast stem cell lines also suggests that ERF may have an FGF2-independent effect during the commitment towards syncytiotrophoblasts. PMID: 28244611
    16. Novel VF-Trap fusion protein on blockage of VEGF and FGF-2 activity to prevent angiogenesis. PMID: 27130666
    17. FGF2 is an extracellular inducer of COUP-TFII expression and may suppress the osteogenic potential of mesenchymal cells by inducing COUP-TFII expression prior to the onset of osteogenic differentiation PMID: 27404388
    18. These results support that controlling the aberrant expression of TGF-beta1 and FGF-2 via inhibition of Wnt/beta-catenin signaling could serve as a potential therapeutic strategy for pulmonary fibrosis. PMID: 27112840
    19. (125)I-bFGF mAb inhibits growth of experimental hepatocellular carcinomas. PMID: 27275095
    20. In vivo, GSPP treatment (200 mg/kg/d) not only inhibited the growth of colon carcinoma, but also inhibited the tumor lymphangiogenesis. Conclusion. GSPP possesses the antitumor ability by inhibiting bFGF-inducing lymphangiogenesis in vitro and in vivo, which may further inhibit tumor lymphatic metastasis. PMID: 27190997
    21. The present study indicates that a broad array of genes associated with functions of the cytoskeleton is significantly dysregulated in the MSC cortex of FGF-2 knockout mice PMID: 26970009
    22. Lack of fibroblast growth factor-2 results in an increased volume of the striatal target area in mice. PMID: 26642808
    23. Low RICTOR expression was detected in quiescent, confluent mouse aortic endothelial cells, whereas high doses of FGF2 induced high RICTOR expression that was associated with strong mTORC2-specific protein kinase Ca and AKT phosphorylation PMID: 26635098
    24. Endogenous FGF2 secreted by trophoectoderm cells regulates protein expression and distribution in trophectoderm cells via FGFR2. PMID: 26378412
    25. IL-1beta promotes FGF-2 expression in chondrocytes PMID: 26811540
    26. FGF2 monoclonal antibody inhibited angiogenesis B16-F10 metastatic melanoma model. PMID: 26655277
    27. Both cell proliferation and hair inductive activity in murine DPCs are maintained by the synergistic effect of fibroblast growth factor 2 and platelet-derived growth factor receptor alpha. PMID: 25975959
    28. These results demonstrated that recombinant human endostatin could in-hibit tumor metastasis by inhibition of the expression of c-Myc and bFGF in gastric cancer tissue as well as by inhibition of angiogenesis PMID: 26125720
    29. Cytosolic LMW FGF2 functions as a negative regulator in RIG-I-mediated antiviral signaling. PMID: 26466960
    30. Data show that, depending of its concentration, FGF2 can be either a positive or negative factor of adipogenesis by regulating the ERK signaling pathway providing a mechanistic basis for FGF2 roles in adipogenesis and development of fat tissues. PMID: 25790378
    31. Trans-regulation of oligodendrocyte myelination by neurons through Arf6-regulated secretion of fibroblast growth factor-2. PMID: 25144208
    32. Data indicate that microbiota-driven fibroblast growth factor 2 (FGF2) and interleukin-17 (IL-17) cooperate to repair the damaged intestinal epithelium. PMID: 26320657
    33. suggest that CD44-positive cells might be a source of cerebellar oligodendrocytes and that FGF-2 plays important roles in their development at an early postnatal stage PMID: 26079890
    34. This study reveals a novel role for the ERK5-MEF2 cascade, linking bFGF-induced PAI-1 expression and subsequent mitogenic processes in lung fibroblasts. PMID: 26032256
    35. Astrocyte-derived bFGF is required for regulation of dopaminergic neurons differentiation of the stem cells and may provide a strategy targeting astrocytes for treatment of Parkinson's disease. PMID: 25517983
    36. both bone morphogenetic protein and Wnt pathways may be involved in mediating the effects of fibroblast growth factor 2 on dental pulp cells PMID: 25158181
    37. FGF2 isoforms modulate bone and phosphate homeostasis via multiple pathways related to bone formation, osteoblast differentiation, and Wnt and Fgf2 signaling. PMID: 25389287
    38. Endostar might exert its anti-tumor effect via suppressing b-FGF-induced angiogenesis and b-FGF-activated MAPK signaling pathway. PMID: 25597785
    39. Basic FGF stimulated mesenchymal stem cell proliferation is NF-kappaB pathway dependent. PMID: 25065316
    40. FGF2 acts as a protective growth factor after lung epithelial injury, and call into question the role of FGF2 as a profibrotic growth factor in vivo. PMID: 24988442
    41. The augmentation of FGF2 expression and reduced optokinetic responses during the resolution of surface vasculopathy may indicate a role for FGF2 in the maintenance of neuroretinal function in OIR/ROP. PMID: 25525167
    42. These studies demonstrate a feedback loop between Klotho depletion and FGF2 activation in renal fibrosis. PMID: 25130652
    43. These findings indicate that the lens epithelium of MCT mice has increased expression of TGFbeta before cataract affection and that changes in the expression of FGF2 as well as TGFbeta may contribute to the progression of the cataract in the mice. PMID: 24279395
    44. FGF2 regulates lymphatic endothelial cell -specific gene expression and suppresses TGFB1 signalling through Smad2. PMID: 24357720
    45. Topical secretoneurin gene therapy accelerates diabetic wound healing by interaction between heparan-sulfate proteoglycans and basic FGF. PMID: 23918206
    46. 131 FGF2-regulated miRNAs during lens fiber cell differentiation PMID: 24142921
    47. In this model the S-domains at the non-reducing ends of the two HS proteoglycan chains are proposed to interact with the FGF2-FGFR2 protein complex. PMID: 24563485
    48. Data suggest that Fgf2-Fgfr2 (fibroblast growth factor 2/fibroblast growth factor receptor 2) signal cascade is involved in stimulating cell proliferation in prostate basal epithelium in absence of androgen replacement therapy following castration. PMID: 23946540
    49. Therefore, constitutive deletion of Fgf2 or Fgfr1 knockdown in oligodendrocyte lineage cells is sufficient to overcome impairment of sensorimotor coordination after cuprizone demyelination. PMID: 23684572
    50. expression of Mll-Ell in myeloid progenitor cells resulted in autocrine production of Fgf2 and Fgf2-dependent cytokine hypersensitivity. PMID: 24089521

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  • 亚细胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 数据库链接：