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  4. Mouse choline acetyltransterase(ChAT)ELISA Kit

Mouse choline acetyltransterase(ChAT)ELISA Kit

  • 中文名称：
    小鼠胆碱乙酰转移酶（ChAT）酶联免疫试剂盒
  • 货号：
    CSB-E17524m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse CHAT ELISA Kit was designed for the quantitative measurement of Mouse CHAT protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    ChatCholine O-acetyltransferase ELISA kit; CHOACTase ELISA kit; ChAT ELISA kit; Choline acetylase ELISA kit; EC 2.3.1.6 ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ChAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 92
    Range % 88-96
    1:200 Average % 87
    Range % 82-91
    1:400 Average % 103
    Range % 99-108
    1:800 Average % 95
    Range % 90-100
  • 回收率：
    The recovery of mouse ChAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-100
    EDTA plasma (n=4) 92 88-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.042 2.136 2.089 1.933
    500 1.407 1.481 1.444 1.288
    250 0.893 0.947 0.920 0.764
    125 0.610 0.658 0.634 0.478
    62.5 0.475 0.463 0.469 0.313
    31.2 0.321 0.305 0.313 0.157
    15.6 0.254 0.249 0.252 0.096
    0 0.159 0.152 0.156 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • 蛋白家族：
    Carnitine/choline acetyltransferase family
  • 数据库链接：