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  4. Mouse factor B,BF ELISA Kit

Mouse factor B,BF ELISA Kit

  • 中文名称：
    小鼠B因子(BF)酶联免疫试剂盒
  • 货号：
    CSB-E04695m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse factor B (BF) ELISA Kit was designed for the quantitative measurement of Mouse factor B (BF) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 μg/mL-20 μg/mL and the sensitivity is 0.078 μg/mL.

  • 别名：
    Cfb ELISA kit; Bf ELISA kit; H2-BfComplement factor B ELISA kit; EC 3.4.21.47 ELISA kit; C3/C5 convertase) [Cleaved into: Complement factor B Ba fragment; Complement factor B Bb fragment] ELISA kit
  • 缩写：
    BF
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.312 μg/mL-20 μg/mL
  • 灵敏度：
    0.078 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse BF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 92-104  
    1:200 Average % 95  
    Range % 91-99  
    1:400 Average % 97  
    Range % 91-105  
    1:800 Average % 102  
    Range % 96-108  
  • 回收率：
    The recovery of mouse BF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 82-94  
    EDTA plasma (n=4) 90 85-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µg/ml OD1 OD2 Average Corrected  
    20 2.712 2.811 2.762 2.608  
    10 2.112 2.212 2.162 2.008  
    5 1.428 1.447 1.438 1.284  
    2.5 0.857 0.866 0.862 0.708  
    1.25 0.524 0.512 0.518 0.364  
    0.625 0.350 0.341 0.346 0.192  
    0.312 0.268 0.263 0.266 0.112  
    0 0.154 0.153 0.154 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    STRING: 10090.ENSMUSP00000025229

    UniGene: Mm.653