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  4. Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

Mouse intestinal fatty acid binding protein,iFABP ELISA Kit

  • 中文名称：
    小鼠肠脂肪酸结合蛋白(iFABP)酶联免疫试剂盒
  • 货号：
    CSB-E08025m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse iFABP ELISA Kit was designed for the quantitative measurement of Mouse iFABP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

  • 别名：
    Fabp2 ELISA Kit; FabpiFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
  • 缩写：
    iFABP
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 102  
    Range % 96-108  
    1:200 Average % 95  
    Range % 91-99  
    1:400 Average % 96  
    Range % 91-102  
    1:800 Average % 86  
    Range % 82-92  
  • 回收率：
    The recovery of mouse iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 82-94  
    EDTA plasma (n=4) 96 90-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.217 2.317 2.267 2.163  
    200 1.367 1.467 1.417 1.313  
    100 0.777 0.797 0.787 0.683  
    50 0.412 0.422 0.417 0.313  
    25 0.265 0.268 0.267 0.163  
    12.5 0.189 0.199 0.194 0.090  
    6.25 0.162 0.165 0.164 0.060  
    0 0.104 0.103 0.104 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • 基因功能参考文献：
    1. This direct comparison provides evidence that LFABP and IFABP have distinct roles in intestinal lipid metabolism; differential intracellular functions in intestine and in liver PMID: 23990461
    2. detected the common as well as sex-differential pathways that are modified due to the loss of Fabp2. These findings suggest that the pathways involved in nutrient and xenobiotic metabolism in the intestine are regulated by sex-specific mechanisms PMID: 22584290
    3. loss of I-FABP renders male mice sensitive to high fat diet-induced fatty liver, and this effect is independent of hepatic L-FABP PMID: 17905650
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Calycin superfamily, Fatty-acid binding protein (FABP) family
  • 组织特异性：
    Expressed in the small intestine. Highest expression levels in the proximal ileum.
  • 数据库链接：