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  4. Mouse lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

Mouse lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

  • 中文名称：
    小鼠脂蛋白相关磷脂酶A2(Lp-PLA2)酶联免疫试剂盒
  • 货号：
    CSB-E08321m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    The mouse Lp-PLA2 Elisa kit is suitable for the quantitative measurement of mouse Lp-PLA2 in serum, plasma, cell culture supernates, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of mouse Lp-PLA2 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision, and recovery, as well as lot-to-lot consistency.

    Lp-PLA2, encoded by the PLA2G7 gene, is a calcium-independent enzyme mainly secreted by macrophages and lymphocytes in atherosclerotic plaques. It is responsible for atherosclerotic plaque progression and instability by promoting inflammation. Lp-PLA2 breakdowns phospholipids in oxidized low-density lipoproteins (ox-LDL) into two proinflammatory and proatherogenic products, lysophosphatidylcholines (lysoPCs) and oxidized nonesterified fatty acids (oxNEFAs), which are involved in endothelial dysfunction and plaque inflammation that promote atherosclerosis. Increased Lp-PLA2 levels are strongly related to atherosclerosis-related diseases, including heart disease and ischemic stroke.

  • 别名：
    Pla2g7 ELISA Kit; Pafah ELISA Kit; Platelet-activating factor acetylhydrolase ELISA Kit; PAF acetylhydrolase ELISA Kit; EC 3.1.1.47 ELISA Kit; 1-alkyl-2-acetylglycerophosphocholine esterase ELISA Kit; 2-acetyl-1-alkylglycerophosphocholine esterase ELISA Kit; LDL-associated phospholipase A2 ELISA Kit; LDL-PLA(2) ELISA Kit; PAF 2-acylhydrolase ELISA Kit
  • 缩写：
    PLA2G7
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-102  
    1:200 Average % 95  
    Range % 84-107  
    1:400 Average % 95  
    Range % 83-102  
    1:800 Average % 96  
    Range % 84-105  
  • 回收率：
    The recovery of mouse Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 86-105  
    EDTA plasma (n=4) 96 88-109  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    500 2.715 2.652 2.684 2.565  
    250 1.817 1.913 1.865 1.746  
    125 1.113 1.165 1.139 1.020  
    62.5 0.600 0.627 0.614 0.495  
    31.2 0.380 0.393 0.387 0.268  
    15.6 0.273 0.292 0.283 0.164  
    7.8 0.192 0.202 0.197 0.078  
    0 0.116 0.122 0.119    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent proinflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in lipoprotein particles and uncontrolled proinflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage.
  • 基因功能参考文献：
    1. Resveratrol downregulates Lp-PLA2 expression by inhibiting oxidative stress via SIRT1 pathway in a mouse model of chronic inflammation. PMID: 28608449
    2. Lp-PLA2 augments the inflammatory response after MI and antagonizes healing by disrupting the balance between inflammation and repair. PMID: 26232205
    3. Plg from mouse plasma contains oxPtdPC adducts that are not affected by the action of Lp-PLA(2), suggesting that linkage to Plg protects oxPtdPCs from metabolism during their transport in the plasma. PMID: 21614211
    4. SAA up-regulates Lp-PLA2 production significantly via a FPRL1/MAPKs./PPAR-gamma signaling pathway. PMID: 23623642
    5. Deletion of Pla2g7 decreases small intestinal polyp and colon tumor incidence in ApcMin/+ mice. PMID: 23361301
    6. Pla2g7 and Tnfrsf21 have been identified as genetic susceptibility to influenza genes in mice. PMID: 22427645
    7. Pla2g7 plays a crucial physiological role in smooth muscle cell differentiation from stem cells, and interactions between Nrf3 and Pla2g7 are essential. PMID: 22247257
    8. The effects of a specific lp-PLA2 inhibitor on atherosclerosis in ApoE-deficient mice and its associated mechanisms, are reported. PMID: 21909350
    9. Studies designed to evaluate the ability of precursor forms of PAF-AH to mature to fully active proteins indicated that the N-terminal end of human and mouse PAF-AH played important and opposite roles in this process. PMID: 20331434
    10. PAF acetylhydrolase activity in the uterus in early pregnancy was not produced locally but probably resulted from the influx of the plasma form of the enzyme PMID: 11833932
    11. deletion of both subunits induces a marked loss of germ cells at an early spermatogenic stage. PMID: 12551946
    12. PAF acetylhydrolases play key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo PMID: 16371369
    13. over-expression of the PAF-AH (I) catalytic subunits induces centrosomal amplification and microtubule disorganization by disturbing intracellular localization of LIS1 PMID: 17903175
    14. inactivation of PAF, produced by TEC, by the overexpression of plasma PAF-AH affects survival, migration, and the angiogenic response of TEC both in vitro and in vivo PMID: 17908960
    15. mouse and human PAF-AHs associate with human HDL particles of different density PMID: 18434304

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  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    Plasma.
  • 数据库链接：

    KEGG: mmu:27226

    STRING: 10090.ENSMUSP00000024706

    UniGene: Mm.9277