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Mouse low density lipoprotein cholesterol (LDL-C) ELISA kit

  • 中文名称：
    小鼠低密度脂蛋白胆固醇(LDL-C)酶联免疫试剂盒
  • 货号：
    CSB-EQ027860MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 缩写：
    LDL-C
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 μg/mL-20 μg/mL
  • 灵敏度：
    0.078 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 86    
    Range % 82-92    
    1:200 Average % 94    
    Range % 91-98    
    1:400 Average % 107    
    Range % 103-114    
    1:800 Average % 89    
    Range % 85-94    
  • 回收率：
    The recovery of mouse LDL-Cspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 88 84-93    
    EDTA plasma (n=4) 91 87-95    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    μg/ml OD1 OD2 Average      
    20 0.105 0.107 0.106      
    10 0.173 0.178 0.176      
    5 0.248 0.255 0.252      
    2.5 0.330 0.345 0.338      
    1.25 0.430 0.443 0.437      
    0.625 0.640 0.682 0.661      
    0.312 0.941 1.011 0.976      
    0 1.752 1.834 1.793      
  • 数据处理：
  • 货期：
    3-5 working days

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