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  4. Mouse neutrophil elastase,NE ELISA Kit

Mouse neutrophil elastase,NE ELISA Kit

  • 中文名称：
    小鼠中性粒细胞弹性蛋白酶(NE)酶联免疫试剂盒
  • 货号：
    CSB-E04804m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    The mouse neutrophil elastase (NE) ELISA Kit is engineered for accurate measurement of mouse NE levels from samples including serum, plasma, cell culture supernates, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the NE content in the sample. The color intensity is positively correlated with NE content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

    NE is a serine protease that plays a causal role in inflammation and is involved in various physiological processes such as the formation of neutrophil extracellular trap and degradation of extracellular matrix and proteins. It is mainly found in neutrophil azurophilic granules. NE can be rapidly released from neutrophils in response to inflammatory stimuli and is therefore regarded as a sign of inflammation and is closely associated with some inflammatory diseases such as bronchiectasis and chronic obstructive pulmonary disease. It also actively participates in cardiovascular disease. NE enhances myocardial damage by triggering excessive inflammation. And many studies have found that high expression of NE in atherosclerotic plaques.

  • 别名：
    Elane ELISA Kit; Ela2Neutrophil elastase ELISA Kit; EC 3.4.21.37 ELISA Kit; Elastase-2 ELISA Kit; Leukocyte elastase ELISA Kit
  • 缩写：
    ELA2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:10Average %88
    Range %82-95
    1:20Average %97
    Range %93-101
    1:40Average %99
    Range %95-106
    1:80Average %93
    Range %87-99
  • 回收率：
    The recovery of mouse NE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-95
    EDTA plasma (n=4)9995-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.849 2.793 2.821 2.627
    102.132 2.222 2.177 1.983
    51.465 1.388 1.427 1.233
    2.50.948 0.929 0.939 0.745
    1.250.643 0.666 0.655 0.461
    0.6250.472 0.442 0.457 0.263
    0.3120.278 0.288 0.283 0.089
    00.198 0.189 0.194 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：组织匀浆液 肺组织匀浆上清

样品信息：小鼠

稀释比：没有稀释

产品评价： 我通过使用CSB-E04804m检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师

靶点详情

  • 功能：
    Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Capable of killing E.coli; probably digests outer membrane protein A (ompA) in E.coli.
  • 基因功能参考文献：
    1. the impact of NE mutations that are typical of human congenital neutropenia in a murine model of in vitro and in vivo neutrophil differentiation PMID: 27942017
    2. Levels of neutrophil elastase (NE) and the nuclear factors CCAAT/enhancer-binding protein alpha (C/EBPalpha) and growth factor-independent 1 (GFI-1) are elevated in hematopoietic stem and progenitor cells from high-fat diet (HFD)-fed mice, but mice lacking either NE or C/EBPalpha are resistant to HFD-induced myelopoiesis. PMID: 28202548
    3. Sivelestat treatment significantly reduced the protein level, neutrophil count, neutrophil elastase activity in bronchoalveolar lavage fluid (BALF). PMID: 28300553
    4. Serine protease required for resistance to microbial infection; contributes to pathogenessis in endotoxic shock PMID: 10714686
    5. Neutrophils from neutrophil elastase-deficient mice show no defect in transendothelial migration PMID: 11994507
    6. results of ultrasound studies using the elastase and AngII apoE in 4 biomechanical parameters: aneurysm diameter, aneurysm volume, circumferential cyclic strain, and blood flow velocity PMID: 26064906
    7. Sirtinol is a novel HNE inhibitor and may have the potential for clinical application in the treatment of inflammatory lung diseases. PMID: 25666548
    8. Increased ELANE activity was associated with development of autoimmune diabetes mellitus in NOD mice. PMID: 25092677
    9. Neutrophil elastase is involved in the induction of microbicidal activities of neutrophils against Trypanosoma cruzi-infected macrophages. PMID: 24599360
    10. contributes to clearance of Aspergillus fumigatus infections PMID: 24054721
    11. In conclusion, sivelestat reduced radiationinduced lung injury in the mice by suppressing neutrophil elastase activity and excessive inflammatory reactions PMID: 23404144
    12. Lung cancer promotion by inflammation is partly mediated by activation of the IL-8/CXCR2 pathway and subsequent recruitment of neutrophils and release of neutrophil elastase. PMID: 24321240
    13. a neutrophil elastase inhibitor prevents bleomycin-induced pulmonary fibrosis in mice PMID: 22441751
    14. During Pseudomonas aeruginosa infection, interleukin (IL)-1beta processing is dependent on neutrophil elastase and not NLRC4 or caspase-1. PMID: 23024281
    15. Neutrophil elastase modulates cytokine expression: contribution to host defense against Pseudomonas aeruginosa-induced pneumonia. PMID: 22927440
    16. NADPH oxidase in antimicrobial host defense against A. fumigatus and B. cepacia, whereas the proteases neutrophil elastase, cathepsin G, and lysosomal cysteine protease cathepsin C/ dipeptidyl peptidase I are dispensable PMID: 22163282
    17. Cathepsin G and neutrophil elastase contribute to lung-protective immunity against mycobacterial infections in mice. PMID: 22461690
    18. Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. PMID: 21979170
    19. This is the first study showing a contribution of neutrophil-derived neutral serine proteases CG and NE to lung-protective immunity against focal pneumonia-inducing serotype 19 S. pneumoniae in mice. PMID: 21911460
    20. The selective sensitivity of G913X Elane granulocytic cells to ER stress provides new and strong support for the unfolded protein response model of disease pathogenesis in severe congenital neutropenia. PMID: 21285438
    21. neutrophil infiltration and neutrophil elastase (NE) activity are elevated in the chronic UVB-irradiated skin of hairless mice, and confirmed the involvement of NE in MMP activation PMID: 20965700
    22. In the absence of a pathogen challenge, neutrophil-derived proteases and nucleosomes contribute to large vessel thrombosis, the main trigger of myocardial infarction and stroke. PMID: 20676107
    23. Since NE is maximally produced in promyelocytes, this protease may play a role in APL pathogenesis by facilitating the leukemogenic potential of PML-RARalpha. PMID: 14636558
    24. neutrophil elastase and cathepsin G are inhibited by PAI-1 mutants PMID: 15131125
    25. Neutrophil elastase has a role in PML-retinoic acid receptor alpha activities in early myeloid cells PMID: 15601827
    26. When challenged intraperitoneally, mice deficient in neutrophil elastase are markedly more susceptible to Klebsiella infection and death. PMID: 15661916
    27. PECAM-1, alpha6 integrins and neutrophil elastase work together to mediate neutrophil transmigration PMID: 15840647
    28. The results of the present study strongly suggest that neutrophil elastase may represent a common pathogenic link between emphysema and fibrosis. PMID: 16045796
    29. Neutrophil elastase depends on serglycin proteoglycan for localization in granules. PMID: 17272511
    30. This study describes for the first time a novel hierarchy among proteases whereby a serine protease NE up-regulates expression of MMPs and cathepsins. PMID: 17442971
    31. Neutrophil elastase and proteinase 3 complexes have different interaction patterns with peptidic substrates. PMID: 18023421
    32. Neutrophil elastase cleaves laminin-332 (laminin-5) generating peptides that are chemotactic for neutrophils. PMID: 18178964
    33. required for neutrophil egression from the vasculature into the alveolar space; interfering with this process leads to neutrophil-related endothelial cell injury. PMID: 18276796
    34. neutrophil elastase might decrease the pulmonary endothelial production of prostacyclin by inhibiting endothelial NO production PMID: 18323746
    35. PR3 and neutrophil elastase enhance neutrophil-dependent inflammation by eliminating the local antiinflammatory activity of progranulin. Our results support the use of serine protease inhibitors as antiinflammatory agents. PMID: 18568075
    36. Neutrophil elastase (NE) degrades the major outer membrane protein F (OmpF); a targeted deficiency in NE negates its antibacterial role in vitro and in a Pseudomonas aeruginosa pneumonia model. PMID: 18802098
    37. during acute peritoneal inflammation, NE expression and activity increase gradually, facilitating leukocyte influx. PMID: 19088179
    38. Evidence for a role of Ela2 in antimicrobial immunity and endotoxic shock PMID: 10714686
    39. Evidence for a role of neutrophil elastase in bleomycin-induced fibrosis PMID: 11451907

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  • 蛋白家族：
    Peptidase S1 family, Elastase subfamily
  • 数据库链接：