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  4. Mouse plasmin-antiplasmin complex,PAP ELISA Kit

Mouse plasmin-antiplasmin complex,PAP ELISA Kit

  • 中文名称：
    小鼠纤溶酶抗纤溶酶复合物(PAP)酶联免疫试剂盒
  • 货号：
    CSB-E08436m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse PAP ELISA Kit was designed for the quantitative measurement of Mouse PAP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

  • 别名：
    N/A
  • 缩写：
    PAP
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %87
    Range %83-94
    1:10Average %97
    Range %92-102
    1:20Average %105
    Range %98-111
    1:40Average %93
    Range %87-99
  • 回收率：
    The recovery of mouse PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-96
    EDTA plasma (n=4)10195-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.245 2.264 2.255 2.072
    1001.805 1.855 1.830 1.647
    501.178 1.155 1.167 0.984
    250.739 0.796 0.768 0.585
    12.50.398 0.421 0.410 0.227
    6.250.312 0.335 0.324 0.141
    3.120.259 0.248 0.254 0.071
    00.188 0.178 0.183 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

你能解释一下用于ELISA抗体的性质吗?是否存在能进行纤溶酶原检测的抗体？

A:
CSB-E08436m试剂盒的目标是复合物。该试剂盒中使用了两种不同的抗体，分别为捕获抗体和检测抗体。它们分别专门针对该化合物的两个部分，且不能检测纤溶酶原。