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  4. Mouse sex hormone-binding globulin,SHBG ELISA Kit

Mouse sex hormone-binding globulin,SHBG ELISA Kit

  • 中文名称：
    小鼠性激素结合球蛋白(SHBG)酶联免疫试剂盒
  • 货号：
    CSB-E08233m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse SHBG ELISA Kit was designed for the quantitative measurement of Mouse SHBG protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 1.3 ng/mL-50 ng/mL and the sensitivity is 0.33 ng/mL.

  • 别名：
    Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
  • 缩写：
    SHBG
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    1.3 ng/mL-50 ng/mL
  • 灵敏度：
    0.33 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-97
    1:2Average %97
    Range %92-103
    1:4Average %85
    Range %81-90
    1:8Average %89
    Range %83-95
  • 回收率：
    The recovery of mouse SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-95
    EDTA plasma (n=4)109104-114
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.344 2.491 2.418 2.370
    271.768 1.822 1.795 1.747
    131.003 1.042 1.023 0.975
    3.30.376 0.365 0.371 0.323
    1.30.175 0.189 0.182 0.134
    00.046 0.049 0.048
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
  • 基因功能参考文献：
    1. SHBG suppresses inflammation and lipid accumulation in macrophages and adipocytes, which might be among the mechanisms underlying the protective effect of SHBG, that is, its actions which reduce the incidence of metabolic syndrome PMID: 30046278
    2. A wave of apoptosis during the pre-pubertal period is required for normal spermatogenesis to develop, and our data indicate that this apoptotic wave may be regulated by ABP and/or androgens. PMID: 12831397
    3. USF transcription factors repress SHBG transcription in Sertoli cells through an interaction with FP4 within its proximal promoter PMID: 15574421
    4. monosaccharide-induced lipogenesis reduced hepatic HNF-4alpha levels, which in turn attenuated SHBG expression. PMID: 17992261
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: mmu:20415

    STRING: 10090.ENSMUSP00000005334

    UniGene: Mm.1431