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  4. Mouse tumor necrosis factor α (TNF-α) antibody ELISA Kit

Mouse tumor necrosis factor α (TNF-α) antibody ELISA Kit

  • 中文名称：
    小鼠肿瘤坏死因子α(TNF-α)抗体酶联免疫试剂盒
  • 货号：
    CSB-EQ023955MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse TNF-α Ab ELISA Kit was designed for the quantitative measurement of Mouse TNF-α Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    antibody for DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
  • 缩写：
    TNF-α Ab
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 80-92  
    1:2 Average % 97  
    Range % 90-105  
    1:4 Average % 87-96  
    Range % 82-96  
    1:8 Average % 93  
    Range % 88-100  
  • 回收率：
    The recovery of mouse TNF-α antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-94  
    EDTA plasma (n=4) 92 87-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.713 2.752 2.733 2.580  
    5 2.212 2.349 2.281 2.128  
    2.5 1.514 1.623 1.569 1.416  
    1.25 0.849 0.864 0.857 0.704  
    0.625 0.502 0.525 0.514 0.361  
    0.312 0.313 0.316 0.315 0.162  
    0.156 0.257 0.270 0.264 0.111  
    0 0.148 0.157 0.153 　  
  • 数据处理：
  • 货期：
    3-5 working days

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