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  4. Mouse urokinase plasminogen activator,uPA ELISA kit

Mouse urokinase plasminogen activator,uPA ELISA kit

  • 中文名称：
    小鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒
  • 货号：
    CSB-E07369m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    CUSABIO's mouse urokinase plasminogen activator (uPA) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse uPA in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of uPA in samples. The color intensity is measured at 450 nm via a microplate reader.

    uPA (PLAU) is a serine protease that cleaves and activates plasminogen, which triggers a proteolytic cascade to regulate extracellular matrix (ECM) proteins. The uPA and uPAR interaction is involved in various cellular activities, including cell proliferation, adhesion, invasion, and survival but is also linked to a broad range of pathological conditions including cancer, atherosclerosis, and kidney disease. In cancer, enhanced levels of the tumor-associated serine protease uPA and its receptor uPAR are linked to tumor progression, metastasis, and shortened survival in patients afflicted with this disease.

  • 别名：
    PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 102
    Range % 96-107
    1:2 Average % 96
    Range % 90-100
    1:4 Average % 95
    Range % 89-100
    1:8 Average % 98
    Range % 92-103
  • 回收率：
    The recovery of mouse uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 93 86-97
    EDTA plasma (n=4) 101 95-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.535 2.435 2.485 2.322
    500 1.918 1.867 1.893 1.730
    250 1.170 1.143 1.157 0.994
    125 0.641 0.633 0.637 0.474
    62.5 0.398 0.385 0.392 0.229
    31.2 0.283 0.289 0.286 0.123
    15.6 0.209 0.214 0.212 0.049
    0 0.166 0.160 0.163 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
  • 基因功能参考文献：
    1. downregulation of uPA could decrease the fertility of male mice, which may be caused by a reduction in sperm motility. PMID: 28298247
    2. data presented here reveal important information about dynamics in uPA by demonstrating how various ligands can modulate uPA activity by mediating long-range conformational changes PMID: 29420634
    3. Lymphangioleiomyomatosis is fatal lung disease associated with germline/somatic inactivating mutations in TSC2 genes. Data suggest lung lymphangioleiomyomatosis lesions and renal angiomyolipomas overexpress uPA; Tsc2-/- (or Tsc1-/-) embryonic fibroblasts from knockout mice express higher uPA levels than wild-type cells. (uPA = urokinase-type plasminogen activator; Tsc = tuberous sclerosis complex protein) PMID: 28972182
    4. The findings suggest that the absence of uPA correlates with increased levels of Runx transcriptional regulators in a way that promotes inflammation-associated carcinogenesis. PMID: 29107070
    5. an intricate link between caveolin-1 and Src kinase-mediated cell signaling and alveolar epithelial cell apoptosis due to loss of SP-C expression through p53 and uPA system-mediated cross-talk, is reported. PMID: 28385810
    6. These studies identify uPA-dependent de-repression of vegfr1 and vegfr2 gene transcription through binding to HHEX/PRH as a novel mechanism by which uPA mediates the pro-angiogenic effects of VEGF and identifies a potential new target for control of pathologic angiogenesis. PMID: 27151212
    7. Significance of the urokinase-type plasminogen activator and its receptor in the progression of focal segmental glomerulosclerosis in clinical and mouse models. PMID: 26846181
    8. GM-CSF and uPA are required for Porphyromonas gingivalis-induced alveolar bone loss in a mouse periodontitis model. PMID: 25753270
    9. Plau deficiency does not worsen controlled cortical impact-induced brain pathology or epileptogenesis caused by TBI. PMID: 26253597
    10. we have firstly shown a fundamental mechanism of urokinase system(uPa and uPAR)-dependent regulation of the trajectory of growth and branching of blood vessels in early embryogenesis and in adults during the repair/regeneration of tissues. PMID: 26863767
    11. Pharmacological inhibition of either uPA or selected MMPs decreased atherosclerosis in transgenic uPA mice. PMID: 25616415
    12. Porphyromonas gingivalis-derived RgpA-Kgp complex activates the macrophage uPA. PMID: 25979345
    13. transgenic alphaMUPA mice, with genetically extended life span, showed a much slower age-related decline in the rate of wound healing than their wild-type counterparts PMID: 25960543
    14. Binding of urokinase to urokinase plasminogen activator receptor promotes dendritic spine recovery and functional outcome after ischemic stroke. PMID: 25339736
    15. our results suggest a role for uPA and uPAR in experimental autoimmune encephalomyelitis pathogenesis PMID: 24120085
    16. Urokinase plasminogen activator is a central regulator of macrophage three-dimensional invasion, matrix degradation, and adhesion. PMID: 24616477
    17. SERPINE2 and PLAU are involved in cumulus expansion and oocyte maturation. PMID: 24023701
    18. Data suggest urokinase-type plasminogen activator (uPA) is involved in susceptibility/resistance to Group A streptococcal infection (GAS); mice expressing human plasminogen transgene are more susceptible to invasive GAS than uPA knockout mice. PMID: 23853591
    19. Mice deficient in urokinase-type plasminogen activator have delayed healing of tympanic membrane perforations. PMID: 23236466
    20. PLAU is particularly important for memory Tregs and PLAU mediates Treg suppressor function via STAT5 and ERK signaling pathways. PMID: 23169000
    21. The data showed that myeloid-derived suppressor cell accumulation is tumor burden-dependent, and that tumor-derived factors such as plasminogen activator urokinase and its receptor play a role in their recruitment. PMID: 23060546
    22. The study establishes the 37- and 70-loops as a unique site for binding to compounds stabilizing the active state of serine proteases. PMID: 22950516
    23. Over-expression of uPA plays a synergistic role in the development of liver injury, inflammation and regeneration during acute HBV infection PMID: 22563169
    24. Data presented here suggest that MMP-9 is secreted as a multiprotein complex by T. gondii infected macrophages, similar to that observed in metastatic cells. PMID: 22177333
    25. u-PA and plasminogen are essential for the phagocytosis of cellular debris by macrophages during liver repair PMID: 22318286
    26. Expression of ALDH1A2, BEX2, EGR2, CCL3 and PLAU are upregulated in Toxoplasma gondiisusceptible C57BL/6 mice. PMID: 22451728
    27. suggest that uPA promotes cell migration by binding to fibulin-5, initiating its cleavage by plasmin, which leads to its dissociation from beta1-integrin and thereby unblocks the capacity of integrin to facilitate cell motility PMID: 22280367
    28. The uPA-uPAR axis has no effect on the formation of Ang II-induced abdominal aortic aneurysms, but uPA deficiency promotes aneurysmal rupture. PMID: 21868698
    29. Urokinase type plasminogen activator, at concentrations found under pathological conditions, reduced pulmonary arterial contractility and increased permeability though the activation of NMDA-R1. PMID: 21617202
    30. uPA acts in a cell signaling manner via binding to its receptor uPAR rather than as a protease. PMID: 21573723
    31. contributes to heterogeneity of macrophages at the border of damaged site during liver repair PMID: 21301782
    32. mechanisms of uPA/uPAR/plasminogen-accelerated atherosclerosis and aneurysm formation PMID: 21536666
    33. The interaction of sM6P/IGF2R with Plg may be an important regulatory mechanism to inhibit migration of cells using the uPA/uPAR system. PMID: 21273553
    34. We propose that u-PA has a protective role in arthritis models with 'wound healing-like' processes following local trauma PMID: 20973954
    35. Early experimental venous thrombus resolution is independent of uPA. PMID: 20886179
    36. study investigated whether host uPA is a crucial protagonist for the TIMP-1-induced modulation of a pro-metastatic microenvironment in the liver PMID: 19863693
    37. uPA/uPAR downregulation suppresses angiogenesis in endothelial cells induced by glioblastoma cell lines PMID: 20805979
    38. Selective abrogation of the uPA-uPAR interaction is associated with suppression of fibrin-associated inflammation. PMID: 20466854
    39. u-PA is required for the full development of systemic arthritis models involving immune complex formation and deposition. PMID: 20196869
    40. uPA plays a critical role in BV-2 microglial cell migration by activating pro-MMP-9, in part by its direct action on MMP-9 and also in part by the activation of plasminogen/plasmin cascade. PMID: 20177776
    41. uPA deficiency can unfavorably modulate both delayed neurodegeneration and neurogenesis but has little effect on post-injury neuronal migration and vascular density. Elevated uPA during the post-injury phase is neuroprotective. PMID: 20026272
    42. MAPK pathways ERK, JNK/SAPK, and P38-MAPK represent a significant component in the regulation of u-PA expression in human prostate cancer. PMID: 11676474
    43. During infection with Cryptococcus neoformans, mice genetically deficient in uPA have a decreased number of recruited effector T cells in the lung, fail to generate a protective T1 profile of cytokines, and display impaired antifungal activity. PMID: 11777975
    44. u-PA-/- mice developed significantly milder disease compared with the relevant wild-type mice PMID: 11891190
    45. fibrinolysis by uPA-dependent plasmin activity plays a fundamental role in skeletal muscle regeneration PMID: 11929773
    46. that u-PA plays important roles in liver regeneration after hepatectomy through control of a transcription factor, c-jun expression. PMID: 11983447
    47. the role of urokinase in the regulation of monocyte/macrophage functions, such as that occurring in inflammatory reactions PMID: 12183060
    48. uPA regulated by heregulin in mouse neoplasms PMID: 12209601
    49. inducible expression reduces fibrosis and mortality after lung injury in mice PMID: 12376355
    50. These results suggest that increasing uPA expression by an appropriate combination of growth factors and ECM components constitutes a possible approach to improving the migration of myogenic cells after transplantation. PMID: 12413883

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    KEGG: mmu:18792

    STRING: 10090.ENSMUSP00000022368

    UniGene: Mm.4183