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  4. Mouse β-hexosaminidase A,β-Hex A ELISA Kit

Mouse β-hexosaminidase A,β-Hex A ELISA Kit

  • 中文名称：
    小鼠β氨基己糖苷酶A(β-Hex A)酶联免疫试剂盒
  • 货号：
    CSB-E04818m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse HEXA ELISA Kit was designed for the quantitative measurement of Mouse HEXA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 μU/mL-200 μU/mL and the sensitivity is 0.78 μU/mL.

  • 别名：
    Hexa ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexosaminidase subunit A ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 μU/mL-200 μU/mL
  • 灵敏度：
    0.78 μU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:10Average %92
    Range %85-100
    1:20Average %93
    Range %87-98
    1:40Average %90
    Range %86-100
    1:80Average %90
    Range %86-98
  • 回收率：
    The recovery of mouse β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9789-101
    EDTA plasma (n=4)9592-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μU/mlOD1OD2AverageCorrected
    2002.542 2.517 2.530 2.386
    1002.073 2.101 2.087 1.943
    501.452 1.464 1.458 1.314
    250.942 0.961 0.952 0.808
    12.50.416 0.402 0.409 0.265
    6.250.325 0.311 0.318 0.174
    3.120.247 0.237 0.242 0.098
    00.146 0.141 0.144
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme S is as active as the isozyme A on the anionic bis-sulfated glycans, the chondroitin-6-sulfate trisaccharide (C6S-3), and the dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A.
  • 基因功能参考文献：
    1. Neu4 is not the only sialidase contributing to the metabolic bypass in Hexa(-/-) mice. PMID: 20862357
  • 亚细胞定位：
    Lysosome.
  • 蛋白家族：
    Glycosyl hydrolase 20 family
  • 组织特异性：
    Ubiquitous. Most abundant in testis, adrenal, epididymis and heart. Low levels seen in the liver.
  • 数据库链接：

    KEGG: mmu:15211

    STRING: 10090.ENSMUSP00000026262

    UniGene: Mm.2284