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  4. Pig Complement 3,C3 ELISA Kit

Pig Complement 3,C3 ELISA Kit

  • 中文名称：
    猪补体蛋白3(C3)酶联免疫试剂盒
  • 货号：
    CSB-E06920p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig complement component 3 ELISA Kit was designed for the quantitative measurement of Pig complement component 3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.625 μg/mL-10 μg/mL and the sensitivity is 0.625 μg/mL .

  • 别名：
    C3 ELISA Kit; Complement C3 [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA Kit; C3bc); Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein ELISA Kit; ASP ELISA Kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 μg/mL-10 μg/mL
  • 灵敏度：
    0.625 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %96
    Range %91-100
    1:200Average %93
    Range %84-103
    1:400Average %95
    Range %91-101
    1:800Average %93
    Range %83-102
  • 回收率：
    The recovery of pig C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9788-108
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    100.083 0.084 0.084
    50.185 0.176 0.181
    2.50.349 0.352 0.351
    1.250.769 0.776 0.773
    0.6251.285 1.292 1.289
    02.140 2.151 2.146
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in post-prandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2.
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: ssc:397072

    STRING: 9823.ENSSSCP00000014399

    UniGene: Ssc.61