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  4. Pig Erythropoietin,EPO ELISA Kit

Pig Erythropoietin,EPO ELISA Kit

  • 中文名称：
    猪红细胞生成素(EPO)酶联免疫试剂盒
  • 货号：
    CSB-E12056p
  • 规格：
    96T/48T
  • 价格：
    ￥4500/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig EPO ELISA Kit was designed for the quantitative measurement of Pig EPO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1 mIU/mL-40 mIU/mL and the sensitivity is 0.5 mIU/mL.

  • 别名：
    EPOErythropoietin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1 mIU/mL-40 mIU/mL
  • 灵敏度：
    0.5 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 83-91  
    1:2 Average % 88  
    Range % 84-93  
    1:4 Average % 97  
    Range % 95-101  
    1:8 Average % 100  
    Range % 97-103  
  • 回收率：
    The recovery of pig EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 95-102  
    EDTA plasma (n=4) 93 90-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml. OD1 OD2 Average    
    40 2.687 2.699 2.693    
    20 1.980 1.879 1.930    
    10 1.105 1.097 1.101    
    5 0.604 0.634 0.619    
    2 0.271 0.293 0.282    
    1 0.132 0.134 0.133    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Could you please advise on the following: 1) what sample types can be used? (plasma, serum, etc)
2) has the kit been validated using porcine samples?
3) was it native or recombinant protein that was tested? Thank you

A:
Thanks for your inquiry.
We tested pig serum and plasma samples before and the test value is
ND--2mIU/ml. Most samples can not be detected out.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
  • 基因功能参考文献：
    1. EPO might play a role as a survival factor or as a mitogen in developing cartilage tissue. PMID: 19908127
    2. Pyruvate-fortified cardioplegia evokes myocardial erythropoietin signaling in swine undergoing cardiopulmonary bypass. PMID: 19767525
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 组织特异性：
    Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • 数据库链接：