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  4. Pig Haptoglobin,Hpt/HP ELISA Kit

Pig Haptoglobin,Hpt/HP ELISA Kit

  • 中文名称：
    猪结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒
  • 货号：
    CSB-E13424p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig Haptoglobin (HP) ELISA Kit was designed for the quantitative measurement of Pig Haptoglobin (HP) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 μg/mL-100 μg/mL and the sensitivity is 0.39 μg/mL.

  • 别名：
    HP ELISA Kit; Haptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 μg/mL-100 μg/mL
  • 灵敏度：
    0.39 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Hpt/HP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 93  
    Range % 89-98  
    1:40 Average % 98  
    Range % 93-103  
    1:80 Average % 95  
    Range % 89-98  
    1:160 Average % 94  
    Range % 87-98  
  • 回收率：
    The recovery of pig Hpt/HP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-102  
    EDTA plasma (n=4) 87 82-94  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ug/ml OD1 OD2 Average Corrected  
    100 1.812 1.724 1.768 1.625  
    50 1.145 1.212 1.179 1.036  
    25 0.715 0.724 0.720 0.577  
    12.5 0.451 0.438 0.445 0.302  
    6.25 0.312 0.322 0.317 0.174  
    3.12 0.255 0.242 0.249 0.106  
    1.56 0.196 0.190 0.193 0.050  
    0 0.142 0.144 0.143    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
  • 基因功能参考文献：
    1. crystal structure of the dimeric porcine haptoglobin-haemoglobin complex determined at 2.9 A resolution PMID: 22922649
    2. This protein is elevated in porcine postweaning multisystemic wasting syndrome. PMID: 15210076
    3. haptoglobin has a role in oxidative status in piglets undergoing weaning-induced stress PMID: 16438801
    4. This study suggested closer links between the release of haptoglobin, Pig-MAP and monocytes compared to the release of AGP, SAA and transthyretin. PMID: 17629575
    5. The haptoglobin was down-regulated with SNPs PMID: 18777092
    6. Presence of haptoglobin alpha 1S subunit is a reponse to respiratory syndrome virus infection. PMID: 18931071

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    KEGG: ssc:397061

    STRING: 9823.ENSSSCP00000002967

    UniGene: Ssc.37