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  4. Pig Interferon β,IFN-β/IFNB ELISA Kit

Pig Interferon β,IFN-β/IFNB ELISA Kit

  • 中文名称：
    猪β干扰素(IFN-β/IFNB)酶联免疫试剂盒
  • 货号：
    CSB-E09890p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig IFN-β/IFNB ELISA Kit was designed for the quantitative measurement of Pig IFN-β/IFNB protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 50 pg/mL-2000 pg/mL and the sensitivity is 25 pg/mL.

  • 缩写：
    IFN-β/IFNB
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    50 pg/mL-2000 pg/mL
  • 灵敏度：
    25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 85-86  
    1:2 Average % 97  
    Range % 92-101  
    1:4 Average % 89  
    Range % 84-93  
    1:8 Average % 95  
    Range % 90-99  
  • 回收率：
    The recovery of pig IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 84 80-88  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 2.897 2.851 2.874    
    1000 2.089 2.072 2.081    
    500 1.196 1.163 1.180    
    250 0.649 0.687 0.668    
    100 0.286 0.293 0.290    
    50 0.144 0.146 0.145    
  • 数据处理：
  • 货期：
    3-5 working days

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