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  4. Pig Nuclear factor-kappa B,NF-κB ELISA Kit

Pig Nuclear factor-kappa B,NF-κB ELISA Kit

  • 中文名称：
    猪核因子-κB(NF-κB)酶联免疫试剂盒
  • 货号：
    CSB-E13448p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig NF-κB ELISA Kit was designed for the quantitative measurement of Pig NF-κB protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    NF-kappa B,Nuclear factor-kappa B
  • 缩写：
    NF-κB
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %87-95
    1:2Average %103
    Range %99-106
    1:4Average %93
    Range %87-98
    1:8Average %91
    Range %88-95
  • 回收率：
    The recovery of pig NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-102
    EDTA plasma (n=4)9086-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.201 2.101 2.151 2.047
    10001.824 1.761 1.793 1.689
    5001.465 1.530 1.498 1.394
    2501.210 1.156 1.183 1.079
    1250.745 0.799 0.772 0.668
    62.50.356 0.341 0.349 0.245
    31.250.248 0.257 0.253 0.149
    00.102 0.105 0.104
  • 数据处理：
  • 货期：
    3-5 working days

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