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  4. Pig Syndecan-1/CD138(SDC1) ELISA Kit

Pig Syndecan-1/CD138(SDC1) ELISA Kit

  • 中文名称：
    猪Syndecan-1/CD138(SDC1)酶联免疫试剂盒
  • 货号：
    CSB-E17413p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig SDC1 ELISA Kit was designed for the quantitative measurement of Pig SDC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 5 ng/mL-320 ng/mL and the sensitivity is 1.25 ng/mL .

  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    5 ng/mL-320 ng/mL
  • 灵敏度：
    1.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %93
    Range %87-102
    1:4Average %97
    Range %92-102
    1:8Average %91
    Range %80-98
  • 回收率：
    The recovery of pig SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9381-98
    EDTA plasma (n=4)9890-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    3202.413 2.395 2.404 2.094
    1602.114 2.004 2.059 1.749
    801.762 1.674 1.718 1.408
    401.434 1.339 1.387 1.077
    201.002 0.953 0.978 0.668
    100.866 0.859 0.863 0.553
    50.535 0.529 0.532 0.222
    00.322 0.297 0.310 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Good morning, I am wondering if you could let me know what the minimum sample volume required for testing is?

A:
Thanks for your inquiry!
CSB-E17413p
The sample volume needed for single well is 100ul. If your sample volume is not enough, then it may lead to false negative. So we suggest you prepare enough sample.
Q:

Is there usually a better signal in serum samples or perfusate samples
taken while on EVLP?

A:
Thanks for your inquiry!
CSB-E17413p
Usually we use this kit to test serum and plasma samples. Regarding perfusate samples on EVLP before,we can not ensure 100%. In theory if the concentration is within the detection range of the kit, it can be detected out. We suggest you do a pretest first.