Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Pig apolipoprotein A1 (Apo-A1) ELISA Kit

Pig apolipoprotein A1 (Apo-A1) ELISA Kit

  • 中文名称：
    猪载脂蛋白A1(Apo-A1)酶联免疫试剂盒
  • 货号：
    CSB-E06834p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig APOA1 ELISA Kit was designed for the quantitative measurement of Pig APOA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 80 μg/mL-2000 μg/mL and the sensitivity is 50 μg/mL.

  • 别名：
    APOA1Apolipoprotein A-I ELISA Kit; Apo-AI ELISA Kit; ApoA-I ELISA Kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA Kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    80 μg/mL-2000 μg/mL
  • 灵敏度：
    50 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %89-103
    1:2Average %90
    Range %85-103
    1:4Average %93
    Range %88-100
    1:8Average %89
    Range %82-97
  • 回收率：
    The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9996-104
    EDTA plasma (n=4)9086-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    20000.342 0.348 0.345
    8000.533 0.483 0.508
    4800.682 0.657 0.670
    2001.021 0.987 1.004
    801.267 1.325 1.296
    01.903 1.954 1.929
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 基因功能参考文献：
    1. Alterations in the Apo A-I pattern is a good indicator of the presence and severity of infectious diseases in the pig.Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. PMID: 25377528
    2. blood level decreases during experimentally induced acute-phase processes in pigs PMID: 15845530
    3. down-regulation of apolipoprotein A-I and A-IV messages in the liver may be mediated by interleukin 6 and tumor necrosis factor-alpha PMID: 15878672
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 组织特异性：
    Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.
  • 数据库链接：

    KEGG: ssc:397691

    STRING: 9823.ENSSSCP00000015994

    UniGene: Ssc.807