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Pig cholecystokinin,CCK ELISA Kit

  • 中文名称：
    猪胆囊收缩素/肠促胰酶肽(CCK)酶联免疫试剂盒
  • 货号：
    CSB-E10131p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig CCK ELISA Kit was designed for the quantitative measurement of Pig CCK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

  • 别名：
    CCK ELISA Kit; Cholecystokinin ELISA Kit; CCK) [Cleaved into: Cholecystokinin-58 ELISA Kit; CCK58); Cholecystokinin-58 desnonopeptide ELISA Kit; (1-49)-CCK58); Cholecystokinin-39 ELISA Kit; CCK39); Cholecystokinin-33 ELISA Kit; CCK33); Cholecystokinin-25 ELISA Kit; CCK25); Cholecystokinin-18 ELISA Kit; CCK18); Cholecystokinin-12 ELISA Kit; CCK12); Cholecystokinin-8 ELISA Kit; CCK8); Cholecystokinin-7 ELISA Kit; CCK7); Cholecystokinin-5 ELISA Kit; CCK5)] ELISA Kit
  • 缩写：
    CCK
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %93-100
    1:2Average %104
    Range %101-107
    1:4Average %91
    Range %85-97
    1:8Average %90
    Range %87-93
  • 回收率：
    The recovery of pig CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9287-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.179 2.198 2.189 2.031
    501.698 1.677 1.688 1.530
    251.136 1.166 1.151 0.993
    12.50.697 0.658 0.678 0.520
    6.250.486 0.497 0.492 0.334
    3.120.383 0.398 0.391 0.233
    1.560.301 0.321 0.311 0.153
    00.157 0.159 0.158
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
  • 基因功能参考文献：
    1. We conclude that only a small fraction of neuronal CCK is nonsulfated. The intracellular distribution of nonsulfated CCK in neurons suggests that they contribute only modestly to the CCK transmitter activity. PMID: 27535680
    2. Cardiac expression of pro-cholecystokinin is cell-specific, which differentiates the expression from that of intestinal endocrine cells and cerebral neurons. PMID: 25627687
    3. This review explores the role of luminal cholecystokinin on the regulation of exocrine pancreatic secretion in 's studies of neonatal pigs. PMID: 15075451
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Gastrin/cholecystokinin family
  • 组织特异性：
    Synthesized in both cerebral cortex and duodenal mucosa.
  • 数据库链接：

    KEGG: ssc:397468

    STRING: 9823.ENSSSCP00000012023

    UniGene: Ssc.717