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Pig free thyroxine (FT4) ELISA kit

  • 中文名称:
    猪游离甲状腺素(FT4)酶联免疫试剂盒
  • 货号:
    CSB-EQF027512PI
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
        FT4(游离甲状腺素):FT4是甲状腺素中的游离形式,是人体代谢功能的重要调节因子之一。它是由甲状腺腺体分泌的主要甲状腺激素之一,与T3一样,对细胞的能量代谢、生长发育、心血管、神经等系统的正常功能都有着重要的影响。FT4的含量水平是甲状腺功能的重要指标,甲状腺功能亢进时FT4水平会升高,甲状腺功能减退时FT4水平则会降低。
        j9九游会登录入口首页生物所提供的Pig free thyroxine (FT4) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测猪 血清、血浆、细胞培养上清、组织匀浆样本中的FT4,其灵敏度为1.08 pmol/L,检测范围为4 pmol/L-64 pmol/L。
     
  • 缩写:
    FT4
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    4 pmol/L-64 pmol/L
  • 灵敏度:
    1.08 pmol/L
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 85-99    
    1:2 Average % 95    
    Range % 87-106    
    1:4 Average % 96    
    Range % 90-110    
    1:8 Average % 91    
    Range % 86-98    
  • 回收率:
    The recovery of pig FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 93-103    
    EDTA plasma (n=4) 96 93-102    
                   
                   
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pmol/L OD1 OD2 Average      
    4 2.771 2.736 2.754      
    8 1.459 1.442 1.451      
    16 0.827 0.865 0.846      
    32 0.575 0.565 0.570      
    64 0.318 0.307 0.313      
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
    FT4指的是游离甲状腺素,是指在血液中不与蛋白质结合的甲状腺素。最新研究发现,FT4水平与代谢综合征、糖尿病、心血管疾病等疾病密切相关。一项针对14,107名美国成年人的研究表明,FT4水平与冠状动脉钙化风险呈正相关关系。此外,一些研究表明,孕期FT4水平异常与胎儿神经发育相关的基因表达水平有关,提示FT4水平对胎儿神经发育的影响。