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Pig interleukin 8,IL-8 ELISA Kit

  • 中文名称：
    猪白介素8(IL-8/CXCL8)酶联免疫试剂盒
  • 货号：
    CSB-E06787p
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL-8是一种促炎性的细胞因子，也称为趋化因子，在细菌、病毒感染或损伤等情况下被活化的炎症细胞产生。它通过作用于白细胞表面的受体，在血管内皮细胞和周围组织引起炎症反应。IL-8在许多炎症性疾病中发挥作用，包括关节炎、哮喘和慢性阻塞性肺疾病等。因此，IL-8也成为了可能的治疗靶点。
        j9九游会登录入口首页生物所提供的Pig interleukin 8,IL-8 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测猪血清、血浆、细胞培养上清、组织匀浆样本中的IL8，其灵敏度为31.25 pg/mL，检测范围为125 pg/mL-8000 pg/mL。
     
  • 别名：
    CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; Alveolar macrophage chemotactic factor I ELISA Kit; AMCF-I ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-99  
    1:2 Average % 90  
    Range % 86-94  
    1:4 Average % 88  
    Range % 84-93  
    1:8 Average % 92  
    Range % 88-97  
  • 回收率：
    The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-104  
    EDTA plasma (n=4) 85 81-90  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    8000 2.662 2.601 2.632 2.460  
    4000 2.215 2.161 2.188 2.016  
    2000 1.498 1.404 1.451 1.279  
    1000 0.871 0.819 0.845 0.673  
    500 0.510 0.507 0.509 0.337  
    250 0.380 0.359 0.370 0.198  
    125 0.271 0.261 0.266 0.094  
    0 0.174 0.170 0.172 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：细胞培养上清液

样品信息：其他

稀释比：没有稀释

产品评价： 结构很好，已发文章

By 高老师

靶点详情

  • 最新研究进展：
    IL8，也称为白细胞介素-8，是一种细胞因子蛋白质，参与炎症和免疫反应。它主要由激活的单核细胞和巨噬细胞产生，在炎症、感染和损伤等条件下被释放。IL8作为趋化因子，可以引导白细胞移动到感染或受损组织，促进炎症反应和免疫细胞的增殖和激活。最近的研究表明，IL8也参与许多其他生物学过程，如细胞增殖、生存、转移和血管新生等。
  • 功能：
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
  • 基因功能参考文献：
    1. This study evaluated the subcellular localization and other functions of TGEV nsp7 protein through analysis of its effects on cell growth, cell cycle progression, interleukin 8 (IL-8) expression, and NF-kappaB activation. PMID: 29521102
    2. The JNK-activated AP-1 subunit c-Jun was critical for the up-regulation of IL-8 expression by PRRSV PMID: 28347884
    3. These results suggest that porcine circovirus 2 induces IL-8 secretion via the TLR2/MyD88/NF-kappaB signalling pathway. PMID: 26581603
    4. Facilitated degradation of the RGS16 by porcine circovirus type 2 ORF3 further enhances NFkappaB translocation into the nucleus through the ERK1/2 signalling pathway and increased IL-6 and IL-8 mRNA transcripts. PMID: 25575706
    5. Suggest that the interplay between TGFbeta-2 and LPS regulates the levels of IL-8 in the immature newborn intestine. PMID: 25147235
    6. downregulated gene expression elite controllers (fraction of human population displaying resistance to HIV infection) PMID: 23751822
    7. Data suggest that MSK1 and MSK2 are the major CREB kinases in fibroblast-like synoviocytes from rheumatoid arthritis patients stimulated with lysophosphatidic acid and that phosphorylation of CREB1 at Ser-133 plays a significant role in IL-8 production. PMID: 24792438
    8. Study presents evidence demonstrating a single species of exotoxin ApxI, derived from A. pleuropneumoniae serotype 10, induces the expression and production of proinflammatory cytokines IL-1beta, IL-8 and TNF-alpha in porcine alveolar macrophages. PMID: 21314908
    9. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
    10. In swine, IL-8, TNF-ALPHA, INOS AND MIP-1BETA were increased during mechanical ventilation in a time-related fashion. PMID: 23102097
    11. Classical swine fever virus NS2 protein promotes interleukin-8 expression. PMID: 21318239
    12. Developmental changes in IL-8 isoforms may serve to minimize its inflammatory effects in the fetus and also provide a mechanism to restore its full activity after birth PMID: 19232500

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 组织特异性：
    Alveolar macrophages.
  • 数据库链接：

    KEGG: ssc:396880

    STRING: 9823.ENSSSCP00000026976

    UniGene: Ssc.658