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  4. Pig thrombin-antithrombin complex,TAT ELISA Kit

Pig thrombin-antithrombin complex,TAT ELISA Kit

  • 中文名称：
    猪凝血酶抗凝血酶复合物(TAT)酶联免疫试剂盒
  • 货号：
    CSB-E13995p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig TAT ELISA Kit was designed for the quantitative measurement of Pig TAT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 缩写：
    TAT
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %84-100
    1:2Average %97
    Range %91-105
    1:4Average %95
    Range %90-108
    1:8Average %95
    Range %82-105
  • 回收率：
    The recovery of pig TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9385-102
    EDTA plasma (n=4)9892-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.178 2.028 2.103 1.897
    20001.582 1.560 1.571 1.365
    10000.980 0.949 0.965 0.759
    5000.558 0.565 0.562 0.356
    2500.370 0.347 0.359 0.153
    1250.296 0.302 0.299 0.093
    62.50.265 0.275 0.270 0.064
    00.200 0.212 0.206 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

我们正在考虑购买该产品CSB-E13995p，我看到方案规定血样中使用肝素锂或K-EDTA作为抗凝血剂。对于TAT等凝血指标，我的实验室一直使用柠檬酸钠进行体外抗凝。这个方法是否测试过？文档中有原因吗?

A:
血浆检测常用EDTA和肝素作为抗凝剂。您说的柠檬酸钠在理论上没有什么作用。请以实际测试结果为准。