Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit

Rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit

  • 中文名称：
    兔子Ⅱ型胶原(Col Ⅱ)酶联免疫试剂盒
  • 货号：
    CSB-E06856Rb
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Rabbit Col ELISA Kit was designed for the quantitative measurement of Rabbit Col protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

  • 别名：
    Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
  • 缩写：
    Col
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma
  • 检测范围：
    6.25 ng/mL-400 ng/mL
  • 灵敏度：
    1.56 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 90-102  
    1:2 Average % 107  
    Range % 97-111  
    1:4 Average % 88  
    Range % 82-92  
    1:8 Average % 98  
    Range % 91-102  
  • 回收率：
    The recovery of rabbit Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 82-94  
    EDTA plasma (n=4) 102 92-107  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    400 2.576 2.528 2.552 2.457  
    200 1.949 1.986 1.968 1.873  
    100 1.421 1.366 1.394 1.299  
    50 0.827 0.811 0.819 0.724  
    25 0.434 0.417 0.426 0.331  
    12.5 0.314 0.302 0.308 0.213  
    6.25 0.234 0.221 0.228 0.133  
    0 0.093 0.097 0.095    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价