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  4. Rabbit Transforming Growth factor β1,TGF-β1 ELISA Kit

Rabbit Transforming Growth factor β1,TGF-β1 ELISA Kit

  • 中文名称：
    兔子转化生长因子β1(TGF-β1)酶联免疫试剂盒
  • 货号：
    CSB-E07000Rb
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    The product CSB-E07000Rb is a sandwich ELISA kit developed to measure levels of rabbit transforming growth factor β1 (TGF-β1) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of TGF-β1 bound in the initial step.

    TGF-β1 (TGFBI) is a pleiotropic cytokine ubiquitously expressed in most tissues with diverse biological functions including senescence, cell proliferation, apoptosis, tumor suppression, differentiation, migration, immunity, osteogenesis, adipogenesis, and wound healing. It importantly contributes to cardiac fibrosis by controlling differentiation, migration, and collagen secretion of cardiac myofibroblasts. TGF-β1 is the most abundant isoform in the mucosa of the gut and may play an important role in the postnatal adaptation of the gastrointestinal tract in suckling animals. It also regulates the intestinal development and function in post-weaning adaptation processes in the intestine of pigs.

  • 别名：
    TGFBI ELISA Kit; Transforming growth factor-beta-induced protein ig-h3 ELISA Kit; Beta ig-h3 ELISA Kit; Kerato-epithelin ELISA Kit; RGD-containing collagen-associated protein ELISA Kit; RGD-CAP ELISA Kit
  • 缩写：
    TGFBI
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.125 ng/ml-50 ng/ml.
  • 灵敏度：
    0.625ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 87-107  
    1:2 Average % 92  
    Range % 87-96  
    1:4 Average % 95  
    Range % 86-105  
    1:8 Average % 96  
    Range % 89-100  
  • 回收率：
    The recovery of rabbit TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 91-102  
    EDTA plasma (n=4) 95 89-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　  
    2.86 0.056 0.058 0.057 　  
    5.7 0.164 0.158 0.161 　  
    11.4 0.382 0.371 0.377 　  
    28.6 0.857 0.867 0.862 　  
    50 1.639 1.621 1.630 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays a role in cell adhesion. May play a role in cell-collagen interactions.
  • 亚细胞定位：
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Located primarily in the epithelium of normal adult cornea, in fetal stromal cells, and both endothelium- and stroma-derived cells in healing corneal wounds. Not expressed in normal adult endothelium and stroma.
  • 数据库链接：