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  4. Rabbit Tumor necrosis factor α,TNFα ELISA Kit

Rabbit Tumor necrosis factor α,TNFα ELISA Kit

  • 中文名称：
    兔子肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
  • 货号：
    CSB-E06998Rb
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        TNF (肿瘤坏死因子，Tumor necrosis factor) TNF是一种由单核细胞、巨噬细胞、T细胞、B细胞等多种细胞合成并分泌的细胞因子，在炎症反应和免疫调节中发挥着重要作用。TNF对炎症反应具有双重作用，既能够诱导和维持炎症反应，又能够抑制炎症反应。此外，TNF还参与细胞凋亡、细胞增殖、细胞分化等生物学过程。在某些疾病状态下，如风湿性关节炎、肝炎、肿瘤等，TNF的表达水平明显升高。
        j9九游会登录入口首页生物所提供的Rabbit Tumor necrosis factor α,TNFα ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测兔血清、血浆、组织匀浆、细胞裂解液样本中的TNF，其灵敏度为19.5 pg/mL，检测范围为78 pg/mL-5000 pg/mL。
     
  • 别名：
    TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
  • 缩写：
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TNFα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 80-92  
    1:2 Average % 95  
    Range % 91-105  
    1:4 Average % 98  
    Range % 92-102  
    1:8 Average % 94  
    Range % 86-98  
  • 回收率：
    The recovery of rabbit TNFα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 87-99  
    EDTA plasma (n=4) 92 87-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    5000 2.905 2.899 2.902 2.789  
    2500 2.366 2.356 2.361 2.248  
    1250 1.639 1.604 1.622 1.509  
    625 0.971 0.939 0.955 0.842  
    312 0.488 0.456 0.472 0.359  
    156 0.275 0.259 0.267 0.154  
    78 0.179 0.171 0.175 0.062  
    0 0.116 0.110 0.113 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 客户评论
  • Tested application: ELISA
    Test sample: Tissue homogenate (Liver and intestine)
    Species: Rabbit
    Review:I tried the kit to determine the best working dilution (starting concentration 100mg/mL). In the case of liver the best result was obtained with 5x-10x dilution, and for intestine the best results were using undiluted sample to 2x dilution.

靶点详情

  • 最新研究进展：
    肿瘤坏死因子（TNF）是一种细胞因子，在免疫调节、炎症和细胞凋亡等生物学过程中发挥重要作用。近年来的研究表明，TNF与各种疾病的发病机制有关，例如炎症性疾病、肿瘤、自身免疫性疾病等。一些新的抗TNF治疗药物也被开发出来，已经用于治疗类风湿性关节炎、克罗恩病等疾病，并获得了一定的疗效。
  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 基因功能参考文献：
    1. TUNEL staining was positively correlated with TNF-a protein expression. Our findings suggest that apoptosis can be induced in the vocal fold epithelium after 120min of modal intensity phonation. In contrast, shorter durations of vibration exposure do not result in apoptosis signaling. PMID: 27577014
    2. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases. PMID: 26125840
    3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
    4. The TNFalpha-evoked Cl- current. PMID: 24084720
    5. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
    6. Hypercapnia increased expression of TNFa and decreased expression of NFKB in acute lung injury models. PMID: 21158126
    7. In the early stages of myocardial ischemia, bone marrow stem cells are mobilized and home to ischemic myocardium with a concomitant increase in expression of cytokines VEGF and TNFalpha. PMID: 20624659
    8. Ammonium perchlorate can increase gene expressions of types I, III collagens, TGF-beta(1) and TNF-alpha in lung of rabbits. PMID: 20937628
    9. TNFalpha may either be directly or indirectly involved in vascular damage following an embolic stroke. Moreover, TNFalpha may mediate some of the detrimental effects of tPA on the vascular compartment PMID: 17673188

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：