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Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit

  • 中文名称:
    兔载脂蛋白B100(Apo-B100)酶联免疫试剂盒
  • 货号:
    CSB-E09805Rb
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
        Apo-B100是一种载脂蛋白,是由APOB基因所编码的一种蛋白质。它是肝细胞中产生的一种大分子蛋白质,其分子量约为512 kDa。与另一种形式的载脂蛋白Apo-B48相比,Apo-B100更为重要,因为它在体内能够结合和运输所有种类的脂质,包括胆固醇、甘油三酯等。
        Apo-B100是低密度脂蛋白(LDL)的主要组成部分,它能够通过血液循环将胆固醇和三酰甘油等脂质从肝脏运输到全身细胞中,并在此过程中与受体结合,促进脂质的吸收和利用。
        血液中Apo-B100的含量可以反映人体内LDL的数量,因此被用作评估心血管疾病风险的指标之一。高水平的Apo-B100与动脉粥样硬化和心血管疾病的发生风险增加密切相关,因此控制Apo-B100的水平对于心血管健康至关重要。
        j9九游会登录入口首页生物所提供的Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测兔血清、血浆、组织匀浆样本中的Apo-B100,其灵敏度为19.5 ng/mL,检测范围为78 ng/mL-5000 ng/mL。
     
  • 别名:
    APOBApolipoprotein B ELISA kit; Fragment ELISA kit
  • 缩写:
    Apo-B100
  • Uniprot No.:
  • 种属:
    Oryctolagus cuniculus (Rabbit)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    78 ng/mL-5000 ng/mL
  • 灵敏度:
    19.5 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 90-102  
    1:200 Average % 92  
    Range % 87-96  
    1:400 Average % 104  
    Range % 98-108  
    1:800 Average % 85  
    Range % 80-89  
  • 回收率:
    The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-102  
    EDTA plasma (n=4) 87 82-92  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    5000 0.092 0.091 0.092    
    2500 0.146 0.138 0.142    
    1250 0.246 0.236 0.241    
    625 0.375 0.360 0.368    
    312 0.524 0.539 0.532    
    156 0.934 0.877 0.906    
    78 1.447 1.334 1.391    
    0 2.432 2.562 2.497    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
        Apo-B100是由APOB基因编码的蛋白质,是低密度脂蛋白(LDL)的主要成分之一。最新研究表明,Apo-B100在冠心病的发病中扮演了重要的角色。研究人员发现,Apo-B100水平升高与冠心病的风险增加有关。此外,一些研究还表明,调节Apo-B100的水平可以改善血脂和血糖代谢,从而降低心血管疾病的风险。
     
  • 功能:
    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 亚细胞定位:
    Cytoplasm. Secreted. Lipid droplet.
  • 组织特异性:
    Detected in liver (at protein level). Highly expressed in liver. Detected at lower levels in jejunum, duodenum and kidney.
  • 数据库链接: