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  4. Rat 4-Hydroxynonenal(HNE)ELISA Kit

Rat 4-Hydroxynonenal(HNE)ELISA Kit

  • 中文名称：
    大鼠4-羟基壬烯酸(HNE)酶联免疫试剂盒
  • 货号：
    CSB-EQ027232RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat HNE ELISA Kit was designed for the quantitative measurement of Rat HNE protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 缩写：
    HNE
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 87  
    Range % 83-92  
    1:200 Average % 96  
    Range % 89-99  
    1:400 Average % 95  
    Range % 91-100  
    1:800 Average % 93  
    Range % 87-98  
  • 回收率：
    The recovery of rat HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-95  
    EDTA plasma (n=4) 98 94-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.118 0.110 0.114    
    500 0.184 0.192 0.188    
    250 0.265 0.273 0.269    
    125 0.474 0.466 0.470    
    62.5 0.625 0.615 0.620    
    31.2 1.129 1.074 1.102    
    15.6 2.182 2.281 2.232    
    0 2.877 2.985 2.931    
  • 数据处理：
  • 货期：
    3-5 working days

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