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  4. Rat Angiopoietin-related protein 4(ANGPTL4) ELISA kit

Rat Angiopoietin-related protein 4(ANGPTL4) ELISA kit

  • 中文名称：
    大鼠血管生成素相关蛋白4(ANGPTL4)酶联免疫试剂盒
  • 货号：
    CSB-EL001712RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    Angptl4Angiopoietin-related protein 4 ELISA kit; Angiopoietin-like protein 4 ELISA kit; Hepatic fibrinogen/angiopoietin-related protein ELISA kit; HFARP) [Cleaved into: ANGPTL4 N-terminal chain; ANGPTL4 C-terminal chain] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, bronchoalveolar lavage fluid, urine
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ANGPTL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 87  
    Range % 83-102  
    1:400 Average % 86  
    Range % 81-90  
    1:800 Average % 92  
    Range % 87-98  
    1:1600 Average % 102  
    Range % 99-105  
  • 回收率：
    The recovery of rat ANGPTL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 89 85-93  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    500 2.634 2.687 2.661 2.561  
    250 2.226 2.268 2.247 2.147  
    125 1.568 1.534 1.551 1.451  
    62.5 0.968 0.943 0.956 0.856  
    31.25 0.527 0.568 0.548 0.448  
    15.62 0.398 0.377 0.388 0.288  
    7.8 0.274 0.289 0.282 0.182  
    0 0.099 0.101 0.100    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis.; Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
  • 基因功能参考文献：
    1. this study represents the first investigation to demonstrate that Angptl4 can predict podocyte injury at earlier stages in minimal change disease PMID: 26352670
    2. GR and FoxO1 are required for Angptl4 transcription activation, and that FoxO1 negatively mediates the suppressive effect of insulin PMID: 24565756
    3. the overexpression of Angptl4 exerts protective, anti-inflammatory and anti-angiogenic effects. It re-presents a novel therapeutic target gene for the treatment of acute lung injury induced by LPS. PMID: 23783408
    4. Data suggest that endothelial lipoprotein lipase (LPL) levels in the heart are altered to maintain FA flux and may exploit angiopoietin-like protein-4 (ANGPTL4). PMID: 22226882
    5. Angptl4 is a novel methylation-silenced gene both in rat and human mammary carcinomas. PMID: 21489049
    6. Angptl-4 is a fasting-induced controller of lipoprotein lipase in adipose tissue, acting extracellularly on the native conformation in an unusual fashion, like an unfolding molecular chaperone PMID: 17088546
    7. ANGPTL4 is a direct GR target that participates in glucocorticoid-regulated triglyceride metabolism. PMID: 19628874

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  • 亚细胞定位：
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 数据库链接：