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  4. Rat Antithrombin III(AT III)ELISA Kit

Rat Antithrombin III(AT III)ELISA Kit

  • 中文名称：
    大鼠抗凝血酶III(AT III)酶联免疫试剂盒
  • 货号：
    CSB-E13885r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Antithrombin III (AT III) ELISA Kit is designed to measure Antithrombin-III (AT III) in rat serum, plasma, and tissue homogenates with remarkable accuracy and sensitivity.

    With a detection range of 39 ng/mL-2500 ng/mL and a sensitivity of 9.75 ng/mL, this quantitative assay can detect even the smallest amount of AT III in your samples. The assay time is only 1-5 hours, and the required sample volume is just 50-100ul. The detection wavelength is 450 nm, ensuring reliable and precise results every time.

    This kit is ideal for researchers investigating the role of AT III in cardiovascular diseases、anticoagulant system research. The sandwich measurement principle allows for accurate quantification of AT III in your samples, making it a valuable tool for any cardiovascular research project. This Rat Antithrombin III (AT III) ELISA Kit has been cited in 2 scientific publications, which speaks to its reliability and popularity within the research community.

  • 缩写：
    AT III
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 ng/mL-2500 ng/mL
  • 灵敏度：
    9.75 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:200 Average % 104    
    Range % 100-112    
    1:400 Average % 84    
    Range % 80-88    
    1:800 Average % 90    
    Range % 85-94    
    1:1600 Average % 97    
    Range % 92-101    
  • 回收率：
    The recovery of rat AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 88 84-92    
    EDTA plasma (n=4) 93 89-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    2500 2.466 2.312 2.389 2.251    
    1250 2.075 1.996 2.036 1.898    
    625 1.664 1.689 1.677 1.539    
    312.5 1.238 1.227 1.233 1.095    
    156 0.702 0.713 0.708 0.570    
    78 0.412 0.392 0.402 0.264    
    39 0.301 0.289 0.295 0.157    
    0 0.135 0.141 0.138 　    
  • 数据处理：
  • 货期：
    3-5 working days

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