Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Apolipoprotein M(APOM) ELISA kit

Rat Apolipoprotein M(APOM) ELISA kit

  • 中文名称：
    大鼠载脂蛋白M(APOM)酶联免疫试剂盒
  • 货号：
    CSB-EL001947RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat APOM ELISA Kit was designed for the quantitative measurement of Rat APOM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    ApomApolipoprotein M ELISA kit; Apo-M ELISA kit; ApoM ELISA kit; Protein Px ELISA kit
  • 缩写：
    APOM
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 96
    Range % 92-100
    1:400 Average % 90
    Range % 85-96
    1:800 Average % 90
    Range % 88-94
    1:1600 Average % 100
    Range % 96-105
  • 回收率：
    The recovery of rat APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 87-94
    EDTA plasma (n=4) 95 90-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    100 2.454 2.460 2.457 2.319
    50 2.045 2.032 2.039 1.901
    25 1.411 1.482 1.447 1.309
    12.5 0.831 0.901 0.866 0.728
    6.25 0.631 0.625 0.628 0.490
    3.12 0.379 0.408 0.394 0.256
    1.56 0.277 0.272 0.275 0.137
    0 0.135 0.140 0.138 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • 基因功能参考文献：
    1. Hyperglycemia-induced downregulation of apolipoprotein M expression is not via the hexosamine pathway. PMID: 26377577
    2. ApoM occurrence in urine may be considered to be a biomarker of acute renal injury. PMID: 23953565
    3. ApoM levels and expression in rats rendered hyperglycemic by short-term glucose infusion, was investigated. PMID: 17556016
  • 亚细胞定位：
    Secreted. Note=Associated with HDL.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Expressed by the liver; secreted in plasma.
  • 数据库链接：

    KEGG: rno:55939

    STRING: 10116.ENSRNOP00000001126

    UniGene: Rn.262