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Rat C-telopeptide of type Ⅱcollagen,CTX-Ⅱ ELISA Kit

  • 中文名称:
    大鼠Ⅱ型胶原C端肽(CTX-Ⅱ)酶联免疫试剂盒
  • 货号:
    CSB-E12963r
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Rat CTX-Ⅱ ELISA Kit was designed for the quantitative measurement of Rat CTX-Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名:
    C-telopeptide of type 2 collagen,CTX-2,_x000D_ C-telopeptide of type II collagen,CTX-II
  • 缩写:
    CTX-Ⅱ
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 85-94  
    1:2 Average % 101  
    Range % 97-104  
    1:4 Average % 91  
    Range % 87-94  
    1:8 Average % 98  
    Range % 95-103  
  • 回收率:
    The recovery of rat CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 91-98  
    EDTA plasma (n=4) 95 91-97  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.232 2.202 2.217 2.076  
    10 1.458 1.447 1.453 1.312  
    5 0.888 0.900 0.894 0.753  
    2.5 0.635 0.621 0.628 0.487  
    1.25 0.375 0.362 0.369 0.228  
    0.625 0.294 0.288 0.291 0.150  
    0.312 0.238 0.227 0.233 0.092  
    0 0.142 0.140 0.141    
  • 数据处理:
  • 货期:
    3-5 working days

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