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  4. Rat Cathepsin K,cath-K ELISA Kit

Rat Cathepsin K,cath-K ELISA Kit

  • 中文名称：
    大鼠组织蛋白酶K(cath-K)酶联免疫试剂盒
  • 货号：
    CSB-E09439r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat CTSK ELISA Kit was designed for the quantitative measurement of Rat CTSK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Ctsk ELISA Kit; Cathepsin K ELISA Kit; EC 3.4.22.38 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 104    
    Range % 100-107    
    1:2 Average % 99    
    Range % 95-103    
    1:4 Average % 85    
    Range % 80-90    
    1:8 Average % 107    
    Range % 103-110    
  • 回收率：
    The recovery of rat cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 86 83-89    
    EDTA plasma (n=4) 95 90-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.801 2.765 2.783 2.682    
    500 2.342 2.204 2.273 2.172    
    250 1.343 1.406 1.375 1.274    
    125 0.679 0.701 0.690 0.589    
    62.5 0.399 0.405 0.402 0.301    
    31.2 0.251 0.242 0.247 0.146    
    15.6 0.166 0.164 0.165 0.064    
    0 0.102 0.100 0.101 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen.
  • 基因功能参考文献：
    1. the present study identified icariin as a novel therapeutic agent in DXM-induced cartilage injury in rats, and revealed that the activation of miR-206 targeting of cathepsin K may be responsible for the chondroprotective efficacy of icariin PMID: 29207029
    2. cathepsin K, in both its secreted and lysosomal forms, may play a role in the degradation of Sertoli cell residual bodies PMID: 14585819
    3. In conclusion, according to its ability to modulate hypotensive effects of kinins, we propose that cathepsin K is a kininase of biological relevance. PMID: 17181996
    4. Comparison of the S2 site between rat and human cathepsin K sequences indicated that two S2 residues at Ser134 and Val160 in rat are varied to Ala and Leu, respectively, in the human enzyme. PMID: 18664521
    5. The expression pattern of Ctsk in osteoclasts in physiological root resorption in the rat molar was determined. PMID: 18974601

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  • 亚细胞定位：
    Lysosome. Secreted. Apical cell membrane; Peripheral membrane protein; Extracellular side.
  • 蛋白家族：
    Peptidase C1 family
  • 数据库链接：

    KEGG: rno:29175

    STRING: 10116.ENSRNOP00000028730

    UniGene: Rn.5598