Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Collagen Type Ⅱ,Col Ⅱ ELISA Kit

Rat Collagen Type Ⅱ,Col Ⅱ ELISA Kit

  • 中文名称：
    大鼠Ⅱ型胶原(Col Ⅱ)酶联免疫试剂盒
  • 货号：
    CSB-E08092r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Col Ⅱ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅱ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

  • 别名：
    Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
  • 缩写：
    Col Ⅱ
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 87  
    Range % 83-92  
    1:200 Average % 94  
    Range % 90-99  
    1:400 Average % 92  
    Range % 88-96  
    1:800 Average % 102  
    Range % 98-105  
  • 回收率：
    The recovery of rat Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 93-96  
    EDTA plasma (n=4) 98 89-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.584 2.599 2.592 2.438  
    500 2.217 2.253 2.235 2.081  
    250 1.765 1.792 1.779 1.625  
    125 1.292 1.264 1.278 1.124  
    62.5 0.789 0.773 0.781 0.627  
    31.2 0.396 0.388 0.392 0.238  
    15.6 0.257 0.269 0.263 0.109  
    0 0.153 0.155 0.154 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价