Rat Creatine Kinase MM isoenzyme,CK-MM ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Rat CK-MM ELISA Kit was designed for the quantitative measurement of Rat CK-MM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 μU/mL-500 μU/mL and the sensitivity is 1.95 μU/mL.
缩写：

CK-MM
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 μU/mL-500 μU/mL
灵敏度：

1.95 μU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	105
	Range %	101-109
1:200	Average %	87
	Range %	84-90
1:400	Average %	102
	Range %	98-106
1:800	Average %	105
	Range %	101-109

回收率：

The recovery of rat CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	87	85-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.